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22001144 NORTHEASTERN NATURALIST 2V1(o2l). :2211,0 N–2o3. 32
A Regional Study of Diversity and Abundance of Small
Mammals in Ohio
John D. Harder1,*, Joy K. Kotheimer1, and Ian M. Hamilton1,2
Abstract - The goal of this study was to obtain information on diversity, abundance, and
distribution of non-volant small mammals in 4 major habitat types in each of 5 regions of
Ohio. We trapped in 31 study areas, representing 39 counties, for 3 consecutive nights for a
total of 38,400 trap nights. We established eight 100-m transects (each with 10 live traps, 20
snap traps, and 20 pitfall traps) per study area in woodland, oldfield, grassland-pasture, or
restored prairie-wetland habitats. We captured fourteen species of small mammals (shrews
and rodents less than 100 g in body mass), but 97% of the 2150 captured consisted of just 4 species:
Microtus pennsylvanicus (Meadow Vole; 31%), Peromyscus leucopus (White-footed
Mouse; 29%), Blarina brevicauda (Short-tailed Shrew; 21%), and Sorex cinereus (Masked
Shrew; 16%). Regional differences in abundance of small mammals (captures/100 trap
nights) and species diversity (H') were not significant (P > 0.05). Seven species of interest
were captured in low numbers (less than 10) and 2 others, Reithrodontomys humulis (Eastern
Harvest Mouse) and Myodes gapperi (Red-backed Vole), were not captured in the course
of the 2-year study.
Introduction
Declining biological diversity is widely recognized as the major challenge in
conservation. Concern is often greatest for losses associated with deforestation
in developing countries, but nations with well-established conservation programs,
such as the United States, are also confronted with this issue. Ecoregion assessments
conducted by the United States Forest Service indicate that 29–46% of
vertebrate species in the US are at risk for population decline (Manley et al. 2005),
and although amphibian declines are widely recognized, evidence warns of declines
in mammalian diversity and populations as well (Entwistle and Stephenson
2002). For instance, Schipper et al. (2008) estimated from a global survey that 25%
of all mammals are threatened with extinction. Unfortunately, such conclusions are
difficult to evaluate because knowledge of the distribution and abundance of mammals
in most states and provinces of North America varies from being current and
detailed for game and endangered species to incomplete and outdated for nearly all
the rest.
In recognition of the challenges to conservation presented by habitat loss and
global climate change, there is a critical need to accurately assess both spatial
and temporal trends in biological diversity in order to develop relevant land-management
and conservation policies (Yoccoz et al. 2001). There is, in fact, sustained
1Department of Evolution, Ecology, and Organismal Biology, 2Department of Mathematics,
The Ohio State University, 318 W. 12th Avenue, Columbus, OH 43210. *Corresponding
author - harder.2@osu.edu.
Manuscript Editor: Thomas French
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interest in studies of mammalian diversity not only in remote tropical regions (Bateman
et al. 2010, Caro et al. 2001, Ojeda et al. 2003) but also in North America and
Europe (McShea et al. 2003, Panzacchi et al. 2010). Long-term studies (Getz et al.
2001, Reed and Slade 2008, Schwartz and Whitson 1987) have advanced our understanding
of community ecology and demography, but they are typically
geographically limited and focused on a few species. Sampling protocols have been
developed for detecting population change for large suites of vertebrate species
based on presence-absence monitoring (Manley et al. 2005), but more detailed,
taxon-specific surveys are also needed to provide species-abundance data for comparison
with results from large-scale surveys.
Efforts in mammalian conservation have traditionally focused on large “charismatic”
species, even though most mammalian species are small in size. For example,
Entwistle and Stephenson (2002) found that only 15% of the papers published in 4
major conservation journals during 1986–1996 featured mammals with a body mass
of less than 1 kg. Moreover, current studies of small mammals typically focus on community
and population biology and seldom provide regional assessments of diversity
and abundance or update systematic collections. For instance, less than 7% of the
specimens of the 6 most common species in museum records (bats, insectivores, and
small rodents) of Ohio were collected after 1985 (Harder 1997). Unfortunately, the
majority of mammalian species lost in the last 400 years (≈75%), as well as those
most likely to become extinct in the near future, are small mammals (Entwistle and
Stephenson 2002). Thus, the need for systematic, regional or statewide surveys of
mammalian diversity in North America is clear.
Our study was designed to assess the distribution, abundance, and diversity of
16 species of non-volant small mammals (i.e., shrews and rodents with a body mass
= less than 100 g) in 4 major habitat types in each of 5 regions of Ohio. This group includes both
common and rare species, but all are susceptible to capture in the array of trap types
used in the study. Because the natural communities and land-use patterns of Ohio are
similar to those in surrounding states, the findings of this investigation have relevance
for the study and conservation of small mammals throughout the region.
Field-site Descriptions
Physiographic provinces of Ohio
Six study areas were established in each of 5 regions in Ohio; these regions represent
a natural subdivision of the state because of how they align with the 4 main
physiographic provinces in Ohio (Powers 2011) (Fig. 1). The northwest region is
primarily low-relief Lake Plains and includes the former Great Black Swamp, the
last major area of the state to be drained and cultivated. It is used intensively for
corn and soybean production and has the highest proportion of land cover under
cultivation (Table 1). Landscape in the northeast region, representing the Glaciated
Plateau, is heterogeneous with glacial moraines and abundant wetlands interspersed
with pasture, row crops, and large tracts of forest. Climate, as evidenced in temperature
and snowfall, is similar to that in northern Pennsylvania and western New York.
The southeast region, almost entirely Unglaciated Appalachian Plateau, is the most
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highly forested region of the state (Table 1), home to the Wayne National Forest
with high hills, sandstone cliffs, and winding streams. The southwest region occupies
the Till Plains and a small portion of Kentucky’s Bluegrass Region in southern
Figure 1. Location of study areas (dots) in each of 5 Regions of Ohio: central (C), northwest
(NW), northeast (NE), southeast SE), and southwest (SW) and their relationship to the 4
major physiographic provinces of Ohio (map modified from Powers 2011), and transect
layout. Small-mammal trapping was conducted on 8 transects at each of the 31 study areas
throughout the State. One Sherman and 2 snap traps were placed at each of 10 stations along
each transect. Three 2.5-m drift fences were placed between the central and 3 peripheral
pitfall traps, forming arms of an array that were separated by arcs of approximately 120°.
Drift fences were omitted on the pitfall trap array placed between stations 5 and 6.
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Adams County. This region features fertile farmland and forested tracts in a gently
rolling topography of glacial moraines and a climate similar to that of northern
Kentucky. The central region is predominantly Till Plains. Headwaters of the Scioto
River and its tributaries originally drained swamp forest in this region, which is
now largely devoted to agriculture in areas outside metropolitan Columbus, Ohio’s
largest city. The 5 regions also correspond to administrative Districts of the Ohio
Division of Wildlife (ODW), which facilitated administrative and logistical support
for the project.
Study areas
We obtained the percent composition of agricultural and natural land-cover
types in 30-m x 30-m cells within an 8-km radius from the center of each study
area by GIS analysis. We obtained the 2006 National Land Cover Data Set (NLCD)
from the United States Department of Agriculture and processed it using the Arc-
GIS Spatial Analyst Extension from Environmental Systems Research Institute. We
buffered and clipped land-cover data to an 8-km radius that included but did not
extend far beyond the margins of the study areas, and summarized data for each
study area by count of the cells in the raster dataset using their classification values
in the NLCD Classification System. We calculated the data as percentages for each
study area.
We sampled each study area, approximately 100 km2, with 8 transects (A–H),
which we placed within 1 of 4 habitat types recognized in this study: 1) woodland,
2) grassland, 3) oldfield, or 4) restored prairie/wetland. These habitat types were
Table 1. Percent cover (mean ± SE) of major natural and agricultural land-use typesA by region of
Ohio. Percentages were computed from the cover type in 30-m x 30-m cells within an 8-km radius
from the center of each study area and averaged by region. Grassland, and to a lesser extent, cultivated
cover types in the NLCD system includes oldfield as recognized in this study. Grassland also includes
restored prairie and wetland (wet meadows).
Region ForestB GrasslandC CultivatedD WetlandE
Central 25.1 ± 7.6 15.6 ± 1.5 59.0 ± 9.6 0.3 ± 0.1
Northwest 12.7 ± 3.5 9.7 ± 3.1 72.0 ± 6.4 5.5 ± 2.7
Northeast 51.3 ± 6.9 17.9 ± 1.9 26.3 ± 4.8 4.5 ± 1.9
Southeast 78.4 ± 5.2 16.5 ± 3.7 4.9 ± 2.1 0.2 ± 0.1
Southwest 44.4 ± 7.1 22.8 ± 1.1 32.5 ± 9.6 0.2 ± 0.1
Statewide 42.4 ± 10.1 16.5 ± 1.1 38.9 ± 9.6 2.2 ± 0.1
A National Land Cover Data Classification System (http://www.mrlc.gov/index.asp). Natural and
agricultural cover types exclude developed areas (commercial, residential, and recreational sites)
and barren ground (sand, pavement), which represent by region the following mean and (range) of
values: Central = 20% (7–60); Northwest = 11% (7–26); Northeast = 13% (8–20); Southeast = 9%
(8–14); and Southwest = 14% (6–22).
BPredominantly (> 95%) deciduous forest but including evergreen forest, mixed forest, and early seral
stage forest.
CPasture and hay but also grassland/herbaceous land cover.
DCultivated crops and orchards.
EBoth woody and herbaceous/emergent wetlands.
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sampled because: a) they are regularly used in published habitat descriptions
and are relevant to habitat preferences of small mammals; b) they represent the
most-common, non-cultivated habitat types on our study areas; and c) they are
well known and are easily identified for reference in future studies. We did not
place transects in cultivated areas because they are disturbed and thus seasonally
variable in development during the field seasons (June–December) of this study.
Also, coverage of this land-use type within study areas varied widely between regions
(Table 1), and allocation of transects to cultivated areas would have reduced
sampling effort in natural habitats expected to harbor a higher diversity of small
mammals. Grassland was agricultural land used for hay production or livestock
grazing. Restored prairie varied from sites seeded predominately with Andropogon
gerardi (Big Bluestem) and Sorgastrum nutans (Indian Grass) to areas more fully
restored to a mixture of grass and prairie forbs. Wetland transects were placed in
wet meadows, typically within 10 m of standing water. Small-mammal collection
records for these transects were grouped for analysis in the prairie/wetland category.
The number of transects placed within each of the 4 habitat types per study area
generally reflected their relative coverage, e.g., the number of woodland transects
in the northeast and southeast regions (Tables 1, 2). However, transect placement
was not proportional to habitat availability in the Southwest Region, where 30 of
48 transects were placed in grassland and oldfield communities, preferred habitats
of Microtus ochrogaster (Prairie Voles) and Reithrodontomys humulis (Eastern
Harvest Mice), the two species of interest in that region.
Methods
Small-mammal sampling
We designated ten species of interest in the design of this study, 2 in each of the
5 regions of Ohio (Table 3). These were species that had not been captured recently
or were otherwise thought to be rare or of restricted distribution in Ohio based
Table 2. Trapping effort in each of 5 regions of Ohio and habitat type is expressed as the number of
transects of 50 traps each that were established and the related number of trap nights.
Regions of Ohio
Central Northwest Northeast Southeast Southwest Total
Transects
Woodland 16 14 28 25 12 95
Grassland 16 18 14 15 16 79
Oldfield 8 11 3 1 14 37
Prairie and wetlandA 8 13 3 7 6 37
Total transects 48 56B 48 48 48 248
Total trap nightsC 8400D 8400 7200 7200 7200 38,400
AIncludes 6 transects in edge habitat.
BAn additional study area was trapped in the northwest region.
CTrap nights per transect per study area is the product of 50 traps x 3 nights = 150, yielding 1200 TN
per study area.
DA study area in Franklin County was trapped for two 3-day sessions, totaling 2400 trap nights.
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Table 3. Non-volant small mammals of Ohio, their historic relative abundance (based on the OWD), and numbers of each species captured in each of the 5 regions
of Ohio and state-wide (Total). % = Percent relative abundanceA . Species richness was lowest in the northwest and highest in the southwest region, but
regional differences in mean (± SE) abundance (captures/100TN) and the Shannon-Weiner index (H') were not significant (P > 0.05).
Species Common nameB % Central NW NE SE SW Total
Sorex cinereus (Kerr) Masked Shrew 7.1 87 92 101 39 23 342
Sorex fumeus Miller Smoky Shrew 7.5 0 0 5 6 2 13
Sorex hoyi Baird Pygmy Shrew 0.1 0 0 0 2 0 2
Blarina brevicauda (Say) Short-tailed Shrew 15.5 124 97 90 77 66 454
Cryptotis parva (Say) Least Shrew 1.1 0 0 0 0 6 6
Peromyscus leucopus (Rafinesque) White-footed Mouse 21.3 163 128 127 122 79 619
Peromyscus maniculatus (Wagner) Deer Mouse 13.7 9 7 0 0 3 19
Reithrodontomys humulis (Aububon and Bachmann) Eastern Harvest Mouse 0.3 0 0 0 0 0 0
Myodes gapperi (Vigors) Southern Red-backed Vole 0.05 0 0 0 0 0 0
Microtus pennsylvanicus (Ord) Meadow Vole 17.2 196 178 131 76 88 669
Microtus ochrogaster (Wagner) Prairie Vole 4.8 0 0 0 0 2 2
Microtus pinetorum (LeConte) Woodland Vole 2.9 0 0 1 3 1 5
Synaptomys cooperi Baird Southern Bog Lemming 2.4 1 0 0 2 0 3
Mus musculus Linnaeus House Mouse 3.2 1 0 0 0 1 2
Zapus hudsonius (Zimmermann) Meadow Jumping Mouse 2.6 1 3 1 4 4 13
Napaeozapus insignis (Miller) Woodland Jumping Mouse 0.5 0 0 1 0 0 1
Totals 100 582 505 457 331 275 2150C
Captures/100 TND 6.9 6.0 6.3 4.6 3.8 5.6
± 1.4 ± 0.9 ± 1.4 ± 1.0 ± 0.7 ± 0.6
Species richness 8 6 8 9 11 14
Species diversity (H')D 1.27 1.25 1.16 1.30 1.40 1.27
APercentage of 13,899 specimens in Ohio Wildlife Database represented by each of 16 species of small mammals, i.e., shrews and rodents less than 100 g in body mass.
BCommon names of 8 species of interest and the number captured in their designated region are boldfaced. The other 2 species of interest in this study,
i.e., Condylura cristata (L.) (Star-nosed Mole) and Spermophilus tridecemlineatus (Mitchill) (Thirteen-lined Ground Squirrel), were not included in this
analysis because they were not sampled effectively with the 4 trap types deployed on the standard transect. Capture data for these species are reported
separately in the text.
CTotal = 2176 with an additional 26 mammals (9 species) that were captured but not included in the analysis: 1 Perascalops breweri (Bachman) (Hairytailed
Mole), 2 Scalopus aquaticus (Rafinesque) (Eastern Mole), 3 Condylura cristata (L.), 1 Sylvilagus floridanus (J.A. Allen) (Eastern Cottontail), 5
Tamias striatus (L.) (Eastern Chipmunk), 9 Spermophilus tridecemlineatus, 1 Tamiasciurus hudsonicus (Erxleben) (Red Squirrel), 1 Mustela nivalis L.
(Least Weasel), 1 Mustela frenata Lichtenstein (Long-tailed Weasel), and 2 unknown (heavily scavenged and not kept).
DCaptures/100 TN (trap nights) and H' (Shannon-Weiner index) were calculated per study area and averaged for presentation by region. ANOVA for captures/
100 TN and H' among regions was computed with data from 6 study areas per region.
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on published data (e.g., Gottschang 1981) and/or low representation in the Ohio
Wildlife Database (OWD), which contains the electronic records of nearly 22,500
voucher specimens of mammals collected in Ohio during the last century and
currently held in 29 collections and museums throughout North America (Harder
1997). Most specimens in the OWD were collected during the 7 decades between
1916 and 1985. The relative abundance of 16 non-volant small-mammal species
(i.e., shrews and rodents with a body mass less than 100 g) in the OWD was a reference for
planning this study and evaluation of the results.
From 17 June–14 December 2004 and 22 June–23 November 2005, we sampled
31 study areas for small mammals: 7 in the northwest region and 6 in each of the
other regions (Table 2). We placed the first 2 study areas trapped in each region in
locations where the designated species of interest had been most recently captured.
Sites for the other 4 study areas per region were selected to provide wide distribution
of study areas and broad coverage of each region. We allocated transects on
all study areas to sample the 4 designated habitat types rather than to focus on the
specialized habitat of any given species. We sampled one new study area per week
(Fig.1). To avoid a seasonal bias in allocation of sampling effort to regions of the
state, we rotated regions in establishment of successive study areas during the two
field seasons.
The trapping-transect design used in this study was intended to expose small
mammals to a wide variety of traps so as to maximize species richness of the
capture. We established each transect with randomized start points in 1 of 4
habitat types, set 10 trap stations at 10-m intervals by pacing on a compass bearing,
and recorded the location of each 100-m transect in UTM coordinates at
stations 1 and 10 (Fig. 1). We set one Sherman live trap (8 x 8 x 22 cm), a Victor
mouse snap trap, and a Museum Special snap trap at each of 10 stations. Traps
were set in surface runways when encountered within approximately 2 m of a
station. We baited traps with a mixture of rolled oats and peanut butter beginning
with the first evening of trapping. We installed five arrays of pitfall traps
(4 traps per array) along each transect, one between every other live-snap trap
station for a total of 50 traps per transect. Holes for pitfall traps were dug with
a golf-cup cutter and lined with a plastic cup (12 cm wide x 15 cm deep). We
set the lip of the cup flush with the ground surface and filled the cup with water
to a depth of about 8 cm. Four of the 5 arrays of pitfall traps per transect were
set with drift fences (0.15 m high x 2.5 m long and anchored with 20-cm gutter
spikes), between the center pitfall trap and each of 3 peripheral traps, which
were separated by approximately 4.4 m in the y-shaped array (Fig. 1). We followed
the recommendation of Kirkland and Sheppard (1994) for use of y-shaped
arrays with drift fences in pitfall trapping, but with a design more similar to that
used by Kalko and Handley (1993). They used pitfall cups of smaller diameter
(11 or 15 cm) with one set in the center and 2 at the ends of 1.2-m drift fences.
Although, installation of pitfall traps is time consuming (about 3-person hrs per
transect), pitfalls are the most efficient trap type for capturing shrews, and they
provide a more accurate estimate of relative abundance of small mammals than
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other trap types (Caceres et al. 2011, Williams and Braun 1983). Traps were set
and checked for 3 days, yielding a total of 1200 trap nights per study area (Table
2). We recorded species, age class (adult/juvenile), sex, reproductive status, and
capture location for each animal captured. Animals captured in live traps were
released unless they could not be readily identified to species, in which case they
were euthanized by cervical dislocation. All procedures involving live mammals
were approved by the Institutional Animal Care and Use Committee at The Ohio
State University.
Rarefaction analysis
This study was designed to maximize species richness of small mammals captured
through use of diverse trap types (4 in all) and intense trapping effort. We
assessed the adequacy of this effort to detect uncommon species by constructing
sample-based rarefaction curves and their confidence intervals (95% C.I.) (Colwell
et al. 2004, 2012) and Chao2 estimates of species richness (95% C.I.) using EstimateS
version 8.2 (Colwell 2005) for each study area. Sample-based rarefaction
curves are expected species accumulation curves that we calculated using analytical
formulae in Colwell et al. (2004). These curves represent the expected number of
species captured for a given number of samples, given the set of species captured
over all samples in a given study area. Chao2 values are estimates of total species
richness, potentially including species that were not captured in any sample. Means
and confidence intervals reported were obtained from 50 randomizations of sample
order. Here we used the Chao2 estimates to assess the adequacy of sampling effort,
rather than as richness estimates per se, by comparing the observed number of species
with the estimated richness. Note: the lower 95% C.I. of the Chao2 estimate
cannot be less than the observed number of species (Chao 1987). We considered
the likelihood of capturing at least 1 additional species, given increased sampling
effort, to be low, if the difference between the observed number of species in a
study area and the upper 95% C.I. for the Chao2 estimate for that study area was
less than 1.
Laboratory analyses
We retained a representative sample (756 specimens) of the capture for identification
of species and preparation of voucher specimens including all 355 long-tailed
shrews (Sorex spp.), 92 Peromyscus spp., and 183 Microtus spp. We deposited
voucher specimens (totaling 712) in the Museum of Biological Diversity, The Ohio
State University, Columbus, OH. Most specimens of Peromyscus spp. (about 90%)
examined in the field were identified as White-footed Mice, based on their capture in
woodland habitat and having a relatively long non-bicolored tail. The sample of 92
Peromyscus spp. retained for laboratory examination was biased. That is, it included
all specimens (n = 25) that showed any indication (i.e., captured in non-forest habitat
and with short, bicolored tail) of being Peromyscus maniculatus (Deer Mouse). Also,
we retained for further study all vole (Microtus spp.) specimens that showed any
indication (pelage and 5 planter tubercles) of being Prairie Vole. The results of Henterly
et al. (2011) suggest that very few specimens of Prairie Vole would have been
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overlooked with this approach. Only 5% (2 of 41) of voles with a Prairie Vole avprla
genotype in their study were identified as Meadow Vole when examined once in the
field, whereas the converse was true for 29% of voles with a Meadow Vole avpr1a
genotype. Mammal carcasses were sprayed with ethanol, placed separately in zipseal
plastic bags, chilled on ice in the field, and stored at -10 ºC until they were thawed
under ultraviolet light in a fume hood for processing.
Small mammals retained as voucher specimens were identified by cranial and
dental features of cleaned skulls. Because identification of White-footed Mice
and Deer Mice, based on external features or cranial/dental morphology, is subject
to substantial error, electrophoresis of salivary amylase is widely recognized as the
only reliable method for identification of the two species collected in Ohio
(Lindquist et al. 2003, Rich et al. 1996). We compared preliminary identification of
White-footed Mice and Deer Mice (based on a sharply bicolored tail and a low taillength-
to-total-body-length ratio) to that obtained from acrylamide gel
electrophoresis of salivary amylase, using the procedures of Bruseo et al. (1999).
We collected saliva by flushing the oral cavity of carcasses with 100 μl of distilled
water, which was collected in a microfuge tube and stored at -10 °C until thawed
and diluted (1:4) for electrophoretic analysis.
Data analysis
We expressed relative abundance of small mammals as captures per 100 trap
nights (TN) per study area. The Shannon-Weiner index (H') was calculated as an
expression of species diversity where H' = -Σ (pi)(ln pi), wherein pi is the proportion
of the total sample that belongs to the ith species and ln is the natural log. We
calculated captures/100 TN and H' per study area. H' were normally distributed
(Shapiro-Wilk test: W = 0.91, df = 31, P > 0.8). Captures/100 TN were square-root
transformed to achieve normality (Shapiro-Wilk test on transformed data: W =
0.99, df = 31, P > 0.9). We performed one-way analysis of variance (ANOVA) for
differences in captures/100 TN and H' among regions with data from 6 study areas
per region, and used Tukey’s post hoc test to check for differences between groups
where appropriate. For each of the 4 most abundant species, we tested effects of
habitat and region on captures with a generalized linear mixed model (GLMM) using
a Poisson distribution and log link. We included captures on each transect as
the dependent variable, habitat and region as a fixed effect, and study area nested in
region as a random effect. We tested habitat and region effects only for the 4 most
abundant species (97% of all captures) because sample sizes for other species (generally
less than 10) were too small for meaningful analysis. Post-hoc pairwise comparisons
were corrected for multiple testing using a sequential Bonferroni correction. We
expressed mean values as a standard error of the mean (SE) and considered differences
statistically significant when P < 0.05.
Results
We captured a total of 2150 small mammals during the two-year study, in which
traps were set for 3 nights on each of 31 study areas statewide yielding a total effort
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of 38,400 trap nights. Although 14 of Ohio’s 16 species of small mammals were
captured, all but 7 were observed in low numbers (less than 10; Table 3), and 97% of the
total capture was comprised of just 4 species: Meadow Vole (31%), White-footed
Mouse (29%), Short-tailed Shrew (21%), and Masked Shrew (16%).
Estimates of species richness
On only 3 study areas (1 in each of central, northwest, and southwest regions) did
the mean Chao2 estimate exceed the observed number of species by more than 1,
and the difference between the observed number of species and the upper 95% C.I.
of the Chao2 richness estimate was less than 1 on 20 of 31 study areas (Fig. 2). This difference
threshold was exceeded in 1 study area in the central region, 2 study areas in the
northeast and southeast regions, and 3 study areas in the southwest and northwest regions.
These results indicate that increased sampling effort in days or transects would
not have increased the number of species detected in most study areas, which is in
accord with the findings of Conard et al. (2008), who found that 3 nights of trapping
are required to reach a stable estimate of species richness at the upper end of the range
of trap densities tested (9–144 trap stations/ha). Our sampling with 4 types of trap,
Figure 2. Number of species trapped (black dots) and mean Chao2 estimate of species richness
(open dots, where different from observed) for each study area, grouped by region of
Ohio. Species richness was estimated using the Chao2 estimate (Colwell et al. 2004) and
calculated in EstimateS Version 8.2 (Colwell 2005). Error bars for species richness estimates
are 95% CI for the mean Chao2 estimates.
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including 20 pitfall traps per transect, was intended to maximize species richness of
the capture. Caceres et al. (2011) concluded that pitfall traps were particularly effective
in this regard, capturing all 14 species known to inhabit their study area, while
Sherman and wire traps captured only 8 (57%).
Abundance and diversity: Variation by region and habitat
Region. Overall abundance of small mammals (all species) varied widely among
study areas from 13.2 captures per 100 TN on a study area in the northeast region
to 2.1/100 TN on one in the southwest region. However, regional differences in total
small-mammal abundance (Table 3) were not significant overall (ANOVA: F =
1.24; df = 4, 26; P > 0.3). Also, regional differences among the 4 most abundant
species (Table 4) were not significant (GLMM: Meadow Vole: F = 0.971; df = 4,
234; P = 0.42; White-footed Mouse: F = 0.809; df = 4, 234; P = 0.52; Short-tailed
Shrew: F = 0.665; df = 4, 234; P = 0.62; and Masked Shrew: F = 1.966; df = 4, 234;
P = 0.10). Although not significant (P = 0.10), regional variation in abundance of
Masked Shrews was high, captures/100 TN being 5 times higher in the Northeast
than in the southwest region (Table 4).
Although small-mammal abundance in the two northern regions was about 45%
higher (P > 0.05) than in the 2 southern regions, the highest species richness for the
designated small mammals in this study (11) was recorded in the southwest region
(Table 3). The southwest region also had the distinction of being the only region
home to all 7 of the most abundant species (Table 4) and to one of the rarest species,
the Prairie Vole (Table 3). However, regional differences in species diversity
(H') were not significant (ANOVA: F = 1.44; df = 4, 26; P > 0.2; Table 3). It should
be noted that our regional comparisons might be biased or compromised because
placement of the first 2 study areas per region were associated with the most recent
capture locations for different species of interest across regions.
Habitat. The relative abundance of small mammals (all species, captures/100
TN) in each of the 4 habitat types viewed statewide (woodland = 5.8, grassland =
6.5, oldfield = 5.4, prairie/wetland 6.0) was similar (P > 0.05). This overall similarity
in small-mammal abundance by habitat reflects the counterbalancing effect of
habitat associations of the most abundant species (Table 5). The effect of habitat
was significant for Masked Shrews, (GLMM—pitfall only: F = 11.380; df = 3, 234;
P < 0.001; all traps: F = 11.288; df = 3, 234; P < 0.001), White-footed Mice (F =
100.053; df = 3, 234; P < 0.001), and Meadow Voles (F = 45.210; df = 3, 234; P less than
0.001), but not for Short-tailed Shrews (F = 2.504; df = 3, 234; P = 0.06). Masked
Shrews were most abundant in restored prairie/wetland (P < 0.05), and White-
Footed Mice were captured most frequently in woodlands (P < 0.5). By contrast,
Meadow Voles were most abundant in grassland-pasture habitat and infrequently
captured in woodlands (P < 0.05; Table 5).
Species of interest
All 10 species of interest were captured infrequently (less than 10 captures), and 3 of
them (Prairie Vole, Microtus pinetorum [Woodland Vole], and Synaptomys cooperi
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Table 4. Mean (± SE)A relative abundance (captures/100 TN) of the 7 most abundant small mammals in 5 regions of Ohio and the number of study areas
in which each species was captured. Regional dif ferences in abundance were not significant (P > 0.05).
Species Central Northwest Northeast Southeast Southwest All regions Study areas
Masked ShrewB
All traps: 1.06 ± 0.46 1.10 ± 0.28 1.40 ± 0.42 0.51 ± 0.21 0.28 ± 0.11 0.88 ± 0.16 28
Pitfall traps only: 2.43 ± 1.07 2.29 ± 0.62 3.37 ± 0.98 1.17 ± 0.48 0.67 ± 0.28 2.01 ± 0.36
Smoky Shrew 0.00 0.00 0.07 ± 0.05 0.08 ± 0.06 0.03 ± 0.03 0.04 ± 0.02 5
Short-tailed Shrew 1.48 ± 0 .38 1.14 ± 0.24 1.25 ± 0.27 1.09 ± 0.32 0.86 ± 0.20 1.17 ± 0.13 31
White-footed Mouse 1.80 ± 0.44 1.50 ± 0.39 1.76 ± 0.38 1.69 ± 0.34 1.11 ± 0.32 1.57 ± 0.17 31
Deer Mouse 0.12 ± 0.03 0.08 ± 0.04 0.00 0.00 0.04 ± 0.40 0.05 ± 0.02 9
Meadow Vole 2.38 ± 0.51 2.12 ± 0.70 1.82 ± 1.41 1.02 ± 0.67 1.23 ± 0.33 1.75 ± 0.34 28
Meadow Jumping Mouse 0.01 ± 0.01 0.04 ± 0.02 0.01 ± 0.01 0.06 ± 0.03 0.06 ± 0.04 0.04 ± 0.01 10
ACaptures/100 TN for each species were calculated for each of 31 study areas and averaged by region.
BMost (90%) were captured in pitfall traps; so, abundance was calculated for all traps (50/transect) and for pitfall traps alone (20/transect).
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[Southern Bog Lemming]) plus Sorex fumeus (Smoky Shrew) and Deer Mouse
were captured in remarkably low numbers (Table 3). Two others, the Eastern Harvest
Mouse and Southern Red-backed Vole, were not captured at all during the
course of our 2-year study.
Only 19 of the 92 specimens (20.6%) of Peromyscus spp. retained for laboratory
examination of skulls and salivary amylase were ultimately identified as Deer
Mice. The sample of Peromyscus spp. retained for laboratory examination included
all specimens (n = 25) that showed any indication of being Deer Mice (see Methods).
These results indicate that only about 3% (19 of all 638 Peromyscus spp.
captured in this study; Table 3) were Deer Mice. Habitat associations support this
conclusion; White-footed Mice were most abundant in forest habitat (Table 5), and
most of the Deer Mice (14/19) were collected in grassland or ol dfield habitats.
Condylura cristata (Star-nosed Mole) and Spermophilus tridecemlineatus
(Thirteen-lined Ground Squirrel) were designated species of interest for this study
because of their limited distribution and low relative abundance in the OWD.
However, they were not susceptible to capture in the array of traps deployed on our
sampling transects and, therefore, they were not included in Table 3 and related
analyses. We captured two Star-nosed Moles in Coshocton County (northern Southeastern
Region) and a third in Williams County (northwest corner of the state) All
3 were captured in pitfall traps set in wetland habitat.
Thirteen-lined Ground Squirrels reach the eastern limit of their continental
distribution in central Ohio, where the existence and size of colonies vary widely
over time. With the aid of local residents, we located robust colonies and established
study areas in Clark and Ross counties (southwest region) and in Licking
and Pickaway counties (central region). Colonies were not identified in Muskingum
County, which is the eastern-most county in Ohio from which specimens have been
collected in the past. We set rat traps (20 per study area) at approximately 20-m
intervals near apparent burrow entrances for 3 nights. We caught three squirrels in
Licking County and 2 at each of the other 3 county sites.
Table 5. Average (± SE) captures/100 TN of the 4 most abundant small mammals in each of the 4
major habitat types sampled in the study. Within a species, values followed by different letters are
significantly different (P < 0.05) from one anotherA.
Habitat types
Species Woodland Oldfield Grassland Prairie/ wetland
Masked ShrewB
Pitfall traps only 1.96 ± 0.38a 1.80 ± 0.49a 1.60 ± 0.31a 4.61 ± 1.04b
All traps: 0.79 ± 0.15a 0.94 ± 0.26a 0.74 ± 0.13a 1.98 ± 0.45b
Short-tailed Shrew 1.39 ± 0.20a 1.30 ± 0.39a 1.25 ± 0.17a 0.76 ± 0.19a
White-footed Mouse 3.28 ± 0.28a 0.94 ± 0.31b 0.32 ± 0.13c 1.24 ± 0.41d
Meadow Vole 0.07 ± 0.06a 1.96 ± 0.42b 3.94 ± 0.57c 2.00 ± 0.39b
ATotal captures per transect (150 TN) compared among habitats using a generalized linear mixed
model with a Poisson distribution and log-link, with study area as a random effect.
BAbundance for this species was calculated for all traps (50/transect) and for pitfall traps alone (20/
transect).
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Discussion
To our knowledge, this is the first large-scale, systematic study of its kind in North
America in several decades. Early information on mammals in Ohio dates from the
publications of Brayton (1882), Kirtland (1838) and most notably Enders (1930),
who trapped mammals on 28 study areas in 10 counties in eastern and southern Ohio,
and the comprehensive review of museum records by Bole and Moulthrop (1942).
The North American Census of Small Mammals, conducted during 1948–1959, includes
reports from cooperators in Ohio (Calhoun 1949). Jorgensen (2004) examined
70 studies of mammalian diversity and microhabitat published between 1969 and
2000 and found that most projects (>50%) involved only 1 or 2 vegetation types, 8 or
fewer species, and relatively small spatial scales. Greatest lineal distances between
study sites in North American studies are typically less than 10 km, and only a few
involve distances in the range of 50 to 150 km (Cudmore and Whitaker 1984, Iverson
et al. 1967, Rickard 1960) or reach 340 km as in Garneau et al. (2012). The 31 study
areas of this investigation were distributed statewide, representing north–south and
east–west lineal distances of 335 and 350 km, respectively (Fig. 1). This wide geographic
distribution of study sites in 4 major habitat types increases the relevance of
our results to concerns regarding the abundance and diversity of small mammals in
other states in the upper Midwest.
Habitat and small-mammal abundance
Three of the 4 most common species showed clear habitat associations (Table 5).
The Short-tailed Shrew was the exception with similar frequency of capture in each
of the habitat types. Although DeCapita and Bookhout (1975) found this shrew
more often than expected in oldfield-pine habitat in southeastern Ohio, our finding
is in accord with conclusions of Bowman et al. (2000), Iverson et al. (1967),
and Osbourne et al. (2005) and supports consensus that the Short-tailed Shrew is a
habitat generalist.
White-footed Mouse. White-footed Mice were found on all 31 study areas,
and although they were well represented in all habitats (except grasslands), their
abundance was highest (P < 0.05) on wooded sites, 2 to 10 times higher than in
the other 3 habitats (Table 5). White-footed Mice occupy diverse habitats throughout
their range in North America but favor wooded areas, even in tallgrass prairie
(Matlack et al. 2008), with vertical density and structural complexity (Drickamer
1990, M’Closkey 1975). Studies of White-footed Mice in Ohio have found highest
densities in the structurally complex edge habitat of smaller forest patches (Anderson
and Meikle 2006) and have demonstrated the importance the annual mast crop
in determining the size of the reproducing population in early spring (Vessey and
Vessey 2007).
Meadow Vole. Statewide, Meadow Voles were captured more frequently than
any other small mammal, except Masked Shrews when calculated for pitfall traps
alone. Being grazers, Meadow Voles are largely restricted to habitats with grass or
herbaceous ground cover (Getz 1985), which is reflected in their highest abundance
(P < 0.05) on transects in grassland habitats and virtual absence from woodland
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2014 Vol. 21, No. 2
sites (Table 5). Meadow Vole abundance was highly variable, particularly in the
northeast region (Table 4), where the number of voles captured per study area
varied from 2 to 105. Spatial variation in vole abundance may be related to sitespecific
conditions, such as cover and food (Getz 1985, Getz et al. 2001), whereas
interaction between food supply and predation appears to be a key factor affecting
population cycles in voles (Krebs 1996). Based on a 25-year demographic study of
Prairie Voles (Getz et al. 2006a) and Meadow Voles (Getz et al. 2006b), the authors
concluded that population fluctuations were initiated by the net effects of periodic
relaxation of predation pressure.
Masked Shrew. Because nearly all Masked Shrews (90%) were captured in pitfall
traps (the highest trap specificity of any species), the abundance of this shrew
should logically be calculated for pitfall traps alone (20/transect), and by this measure,
the abundance of Masked Shrews (2.01/100 TN, in all regions) ranked higher
than that for all other species (Table 4). However, it should be noted that because
most Masked Shrews were captured in pitfall traps, which can function as repeating
traps (i.e., capturing more than one animal per trap night), data on captures/100 TN
might under-represent the trapping effort (TN) and thus overexpress the relative
abundance of this species. Masked Shrews were trapped in 16 counties where they
had not been previously recorded, and 5 of those counties were well outside the
previously published range in northern and south-central Ohio (Gottschang 1981).
Masked Shrews were also the most abundant small mammal in several other study
sites in North America including Manitoba (Wrigley et al. 1979), Prince Edward
Island (Hartling and Silva 2003), southern Pennsylvania (Kirkland and Findley
1999), and West Virginia (Osbourne et al. 2005).
Masked Shrews are found in a variety of habitats that provide a mesic microclimate
(Brooks and Doyle 2001, Menzel et al. 1999, Wrigley et al. 1979). Our data
suggest that restored prairie and wetlands provide favorable, mesic habitat for the
Masked Shrew in Ohio where it reaches the southern limit of its distribution in eastern
North America, outside Appalachia. Masked Shrews have high metabolic and
water-turnover rates (Poppitt et al. 1993, Speakman 1997), which appear to limit
their southern distribution into warmer, drier climates of North America (Churchfield
1990, Ochocinska and Taylor 2005) and favors their southernmost distribution
in cool, moist locations at high elevations of the Appalachian Mountains (Brannon
2002, Ford et al. 2001). Similarly in this study, abundance of Masked Shrews was
5 times higher in the Northeast than in the Southwest Region (P = 0 .10; Table 4).
Northeast Ohio is characterized by lower mean summer temperatures than southwest
Ohio (about 3.6 °C less) (NOAA 2010), which might contribute to cool mesic
habitats favorable to shrew populations during the growing season.
Smoky Shrew. We captured 13 Smoky Shrews, all on study areas in the Glaciated
Plateau or Unglaciated Appalachian Plateau (Fig. 1), confirming the observations
of Gottschang (1981). All Smoky Shrews were captured in pitfall traps set in
wooded areas, and although our sample is too small for statistical analysis, this
pattern is consistent with a specialized habitat preference for shady, damp woods
with leaf litter and decomposing logs found in Ohio (Gottschang 1981), North
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Carolina (Brannon 2002), and Indiana (Cudmore and Whitaker 1984). The relative
abundance (captures/100 pitfall TN in woodland habitat) of Smoky Shrews in our
study (0.35) is similar to that (0.36) recorded in southern Indiana by Cudmore and
Whitaker (1984), who set pitfalls (1000-ml plastic beakers) along natural obstructions
(logs, rock faces) in suitable forest habitat, but lower than the 5.0 recorded by
Brannon (2002) in North Carolina and the 1.50 recorded by Kirkland and Findley
(1999) in southern Pennsylvania. Both of these latter studies employed y-shaped
pitfall arrays in lowland forest habitat. Additional, targeted surveys designed to
sample the restricted habitat of Smoky Shrews with pitfall trap arrays are needed
to fully assess the status of the species in Ohio.
Small-mammal diversity
Statewide species diversity (H' = 1.27) was lower than the H' of 1.78 for small
mammals trapped in riparian forests in West Virginia (Osbourne et al. 2005), and
the H' of 1.57 from a sample of 265 trapped in forests southern Pennsylvania (Kirkland
and Findley 1999), but similar to the H' of 1.33 from small mammals trapped
in the Catskill region of New York (Garneau et al. 2012).
Status of small mammals in Ohio
This study was designed to assess the status of 16 species of small mammals in
Ohio including the abundance and distribution of 10 species of interest, which may
now be placed in 1 of 2 groups. The first group of 5 (Least Shrew, Thirteen-lined
Ground Squirrel, Sorex hoyi [Pygmy Shrew], Star-nosed Mole, and Napaeozapus
insignis [Woodland Jumping Mouse]) were, prior to this study, considered rare,
uncommon, or restricted in distribution (according to Gottschang 1981, Svendson
1976, and their relative abundance in the OWD), and the last 3 were listed as species
of concern by the ODW, Department of Natural Resources (ODW 2012). No
evidence was obtained in this study that would suggest a change in the conservation
status for any of the 5 species in this group.
The second group of 5 species of interest includes 2 (Eastern Harvest Mouse
and Southern Red-backed Vole) that were, prior to this study, thought to be very
rare, if not extirpated from Ohio, whereas the other 3 (Prairie Vole, Woodland Vole,
and Southern Bog Lemming) were considered to be of moderate abundance (Gottschang
1981), each comprising >2% of all small-mammal specimens in the OWD.
Southern Red-backed Vole. The Southern Red-backed Vole was last recorded in
far eastern Ohio (Jefferson County) in 1960 (OWD), and despite our trapping in the
general area of the last 2 known capture locations, none was captured in this study.
In fact, only 7 specimens have ever been collected in Ohio, and 6 of these were captured
in 1926–1929 from sites in eastern Ashtabula County that were subsequently
flooded by the Pymatuning Reservoir (Bole and Moulthrop 1942). Although the
Southern Red-backed Vole is abundant in northeastern states bordering Ohio, it is
listed as extirpated from Ohio (ODW 2012), which is in accord with the conclusion
of Bole and Moulthrop (1942) and Gottschang (1981).
Eastern Harvest Mouse. The Eastern Harvest Mouse was not captured in this
study, but it does not appear to be extirpated from Ohio. It was previously reported
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2014 Vol. 21, No. 2
in 9 counties in southern Ohio, and it was captured on 2 of 5 units during a year-long
(12-month) survey of the Hopewell Culture Historic National Park near Chillicothe
in south-central Ohio (Vick 2004). Our procedures probably were not sufficiently
targeted or intense to detect this species on most study areas. However, this species
is rare, spotty in distribution, and not easily trapped (Gottschang 1965), and Ohio
lies at the northern limit of its distribution. It was first captured in Adams County
(borders the Ohio River) in 1929 (Bole 1932), and prior to the recent captures near
Chillicothe, captures had not been reported since 1971. It currently is listed as
threatened in Ohio (ODW 2012).
Woodland Vole. Even though 65 transects (9750 TN) were placed in woodland
habitat in the northeast, southeast, and southwest regions, only 5 Woodland Voles
were captured (Table 3). We might have captured more had our procedures been
targeted for this species, e.g., setting pitfall traps in subsurface burrows or along
natural obstructions, as in the study of Cudmore and Whitaker (1984), who captured
69 Woodland Voles (in 29,967 TN) in a study of shrews in southern Indiana. Woodland
Voles were not captured in the central or northwest regions, which supports
Gottschang’s (1981) conclusion that this vole is absent from northwestern Ohio.
Although Woodland Voles are widely distributed throughout eastern United States,
the distributional pattern in Ohio might be related to low forest cover and extensive
cultivation in the northwest region (Table 1) or perhaps soil texture as in orchards
in Pennsylvania (Fisher and Anthony 1980).
Southern Bog Lemming. The status of this species in Ohio remains unclear.
The OWD contains a moderate number of records of Southern Bog Lemmings
(328 or 2.4% of total small mammals), with captures recorded from nearly half
(40 of 88) of the counties and all 5 regions of the state, but despite our trapping
on 79 transects (11,850 TN) in grassland habitat (favored by bog lemmings in the
eastern Midwest), we captured only 3 individuals. The Southern Bog Lemming
is known, in the eastern part of its range, to be rare or at least spotty in distribution
and difficult to capture (Gottschang 1981, Linzey 1983). We set our traps in
runways, within constraints of our transect protocol, but we seldom observed the
bright green droppings characteristic of this species. No doubt more targeted procedures
would have captured more Southern Bog Lemmings, but the low number
recorded in our study might also reflect competitive exclusion by Meadow Voles.
Linzey (1984) provided convincing evidence of this in Virginia, and a decline of
Southern Bog Lemmings was associated with a range extension of Meadow Voles
in Kentucky (Krupa and Haskins 1996). Thus, future studies should not only address
the status of this species of concern in Ohio but also explore its ecological
relationship with Meadow Voles.
Prairie Vole. Perhaps the most striking manifestation of low species diversity
of small mammals observed in this study is the dominance of Meadow Voles and
White-footed Mice, which represent 96.6% of all rodent captures. The Prairie Vole
is the seventh-most abundant species of small mammal in the OWD with records
from 30 counties, and it had once been considered to be fairly common in the
southern half of Ohio. In fact, it was equal in abundance (377 captures) to Meadow
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Voles (303 captures) in one study area near Dayton (DeCoursey 1957). However,
despite our deployment of a large number of transects in grassland and prairie/wetland
habitats in the central and southwest regions, totaling 6900 TN, only 2 Prairie
Voles were captured during the entire study, both in Adams County in the southwest
region. By comparison, 35 Prairie Voles (and 7 Southern Bog Lemmings) were
captured in lines of snap-back mouse traps (5538 TN) set in dry oldfield habitat in
western Indiana (Whitaker et al. 2007). Prairie Voles reach the eastern limit of their
distribution in eastern Ohio, western Pennsylvania, and West Virginia. Having been
first recorded in Ohio in 1921 (Henninger 1921), this vole now appears to persist in
very low numbers in Ohio, and it is listed as a species of concern (ODW 2012).
Deer Mouse. Our conclusion that only about 3% of all Peromyscus spp. captured
in this study were Deer Mice is noteworthy with reference to records in the
OWD, wherein 39% (1903 of 4851) of the specimens of Peromyscus spp. were
recorded as Deer Mice. We did not sample cultivated areas, and they are known to
harbor substantial populations of Deer Mice and Mus musculus (House Mouse) in
Illinois (Getz and Brighty 1986) and Indiana (Whitaker 1967), which best explains
the capture of only 2 specimens of the latter (Table 3). Whitaker (1967) found the
abundance of Deer Mice in several cropland types (wheat, corn, soybean) to be
equivalent to or 2–3 times higher than in weedy or grassy fields. Previous studies in
Ohio have shown Deer Mice to be relatively abundant in grassland and oldfield habitats
(DeCoursey 1957, Gottschang 1981). Birch (1977) found that most (57 of 74)
of the Peromyscus spp. captured in oldfield and grassland habitats in south-central
Ohio were Deer Mice (rigorously indentified by discriminate analysis of morphometrics).
But, despite intensive sampling in grassland and oldfield habitats (11,850
and 5500 TN, respectively), we captured only 19 Deer Mice, and this species was
absent in samples collected in 2 of 5 regions (Table 4). Nevertheless, because we
did not sample cultivated fields, we cannot estimate the overall relative abundance
of Deer Mice in the state. Additional, targeted surveys employing electrophoretic
or molecular techniques for identification of Deer Mice are needed to resolve the
status of this species in Ohio.
Star-nosed Moles and Thirteen-lined Ground Squirrels. Since all 3 Star-nosed
Moles captured were caught in wetlands, future efforts to monitor this species of
concern (ODW 2012, 2013) should focus on wetland habitat in northern Ohio. Our
capture results for Thirteen-lined Ground Squirrels and earlier observations cited
in Gottschang (1981) indicate that the eastern range limit of this species in central
and southeastern Ohio has not changed appreciably in the last 30–50 years.
Summary and Conclusions
Twenty-three species of mammals were captured in this study, 14 of which were
small mammals (shrews and small rodents less than 100 g in body mass) and the focus
of our analysis; 97% of 2150 captures of small mammals was represented by just
4 species: Meadow Vole (31%), White-footed Mouse (29%), Short-tailed Shrew
(21%), and Masked Shrew (16%). Species richness varied from 6 in the northwest
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2014 Vol. 21, No. 2
region to 11 in the southwest region. Rarefaction analysis indicated that additional
trapping effort would not have increased the number of species detected in most
study areas. Regional differences in abundance of small mammals (captures/100
trap nights) and species diversity (H') were not significant (P > 0.05). However,
regionally restricted distributions were confirmed for the Smoky Shrew and Woodland
Vole. This study also confirmed well-known habitat preferences for Whitefooted
Mice and Meadow Voles and provided the first evidence of the preferred
habitat of Masked Shrews in Ohio: restored prairie and wetland.
Our systematic transect-sampling procedure might be considered a coarse filter
that is well suited to sampling common to moderately abundant mammals (e.g.,
the 7 species in Table 5, except Smoky Shrew) in major habitat types, but it might
not detect or, in particular, effectively estimate relative abundance of rare species,
especially habitat specialists or species that do not readily enter traps. This category
would include most of our designated species of interest except Deer Mice (absence
of samples from cultivated habitat, notwithstanding) and Prairie Voles.
Four rodent species of questionable status in Ohio (Eastern Harvest Mouse,
Prairie Vole, Southern Bog Lemming, and Deer Mouse) live in grassland or oldfield
communities, which suggests the quantity or quality of these habitats in Ohio may
be insufficient to support large populations of these species. A meta-analysis of
studies of South African grasslands indicated that small-mammal species richness
and diversity declines with habitat degradation and that generalist rodent species
dominate community numbers at low ecological integrity (Avenant 2011). Changing
agricultural land-use patterns in Ohio, such as the 25% reduction of acreage
devoted to hay fields and an 11% increase in that used for corn and soybean production
during 1973–2004 (NASS 2011), might be associated with a general decline
in small-mammal habitat. Additional research is needed to confirm the status of
grassland and oldfield species and to reveal the underlying causes of any changes
in Ohio as well as in other states in the southern Great Lakes region.
Acknowledgments
We are grateful to David Lowell and Matthew Carney for their invaluable assistance
during 2 rigorous field seasons and Travis Brown for his outstanding contribution to the
project in preparation of voucher specimens. We thank Karen Treadway, Amanda Prouty,
Brad Ryan, Brad Condo, and Kyle Tolle for technical assistance; Joni Lung, Adam Andrews,
Travis Fuller, and Brad Graley for field work; and Jimmy Chiucchi for statistical
analysis. Donovan Powers, ODNR, created Figure 1 and provided output from the NLDCL
for percentage cover types for the 31 study areas. Christine Anderson reviewed a draft of
the manuscript. We relied heavily on Ohio Division of Wildlife personnel and private landowners,
too numerous to mention individually, who hosted our fieldwork and assisted in
identifying suitable trapping sites as did administrators and field personnel with the Ohio
State Parks, Ohio State Forests, Wayne National Forest, Ottawa National Wildlife Refuge,
Columbus Franklin County Metro Parks, The Nature Conservancy, and the National Aeronautics
and Space Administration, Plum Brook Station. Carolyn Caldwell, ODW, provided
administrative support and valuable consultation and encouragement throughout the course
of the study. This project was funded by the Ohio Division of Wildlife, ODNR.
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