New Host and Geographic-Distribution Records for
Helminth and Arthropod Parasites of the Southern Toad,
Anaxyrus terrestris (Anura: Bufonidae), from Florida
Chris T. McAllister, Charles R. Bursey, Matthew B. Connior, Stanley E. Trauth, and Lance A. Durden
Southeastern Naturalist, Volume 14, Issue 4 (2015): 641–649
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C.T. McAllister, C.R. Bursey, M.B. Connior, S.E. Trauth, and L.A. Durden
22001155 SOUTHEASTERN NATURALIST 1V4o(4l.) :1644,1 N–6o4. 94
New Host and Geographic-Distribution Records for
Helminth and Arthropod Parasites of the Southern Toad,
Anaxyrus terrestris (Anura: Bufonidae), from Florida
Chris T. McAllister1,*, Charles R. Bursey2, Matthew B. Connior3,
Stanley E. Trauth4, and Lance A. Durden5
Abstract - We collected 18 Anaxyrus terrestris (Southern Toad) in March and May 2014
and July 2015 from Topsail Hill Preserve State Park, Walton County, FL, and examined
them for helminth and arthropod parasites. Fourteen toads (78%) were infected or infested
with parasites as follows: 2 (11%) with Megalodiscus temperatus, 1 (6%) with tetrathyridia
of Mesocestoides sp., 3 (17%) with Cylindrotaenia americana, 2 (11%) with 3rd-stage larval
Physaloptera sp., 9 (50%) with Cosmocercoides variabilis, 10 (55%) with Oswaldocruzia
pipiens, and 2 (11%) with larval Hannemania hegeneri chigger mites. We observed multiple
infections/infestations of helminths and/or arthropods in 7 (39%) of these hosts. Herein, we
report 5 new host and 2 new geographic-distribution records.
Introduction
Anaxyrus terrestris (Bonnaterre) (Southern Toad) is a common toad that has
highly pronounced cranial crests and knobs and is found from extreme southern
Virginia to the Mississippi River, west to Louisiana and south through Florida to
the lower Keys. There is also a disjunct population in the Upper Piedmont and Blue
Ridge of extreme western South Carolina (Conant and Collins 1998). The Southern
Toad is particularly abundant in sandy habitats in wooded and open areas where
it becomes active at dusk and forages into the evening. Although a great deal of
information is available on the natural history of the Southern Toad (Blem 1979),
comparatively little is known about its helminth parasites (see Dickey 1921; Jewell
1916; Pryor and Greiner 2004; Sears et al. 2012; Walton 1938, 1940). Here, we
report new host and distributional records for some parasites of Southern Toads
studied in Florida.
Methods
We collected 18 juvenile and adult Southern Toads by hand on 28 March
(n = 1), 3 May 2014 (n = 9), and 27 July 2015 (n = 8) (12 males, 6 females, mean
± 1 SD snout-vent length [SVL] = 56.0 ± 20.1, range = 30–100 mm) from Topsail
1Science and Mathematics Division, Eastern Oklahoma State College, Idabel, OK 74745.
2Department of Biology, Pennsylvania State University-Shenango Valley Campus, Sharon,
PA 16145. 3Life Sciences, Northwest Arkansas Community College, Bentonville, AR
72712. 4Department of Biological Sciences, Arkansas State University, State University,
AR 72467. 5Department of Biology, Georgia Southern University, 4324 Old Register Road,
Statesboro, GA 30458. *Corresponding author - cmcallister@se.edu.
Manuscript Editor: David Bruce Conn
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Hill Preserve State Park, Walton County, FL (30°22'11.3"N, 86°17'48.1"W). We
placed all specimens in individual bags on ice and transported them to the laboratory
within 48 hr for necropsy. Following procedures for humane treatment of
amphibians (HACC 2004), we overdosed the toads with a concentrated Chloretone
solution (Lab Connections, St. Augustine, FL), and examined their tegument
for ectoparasites. We used dissecting scissors and fine forceps to carefully
remove Hannemania sp. (chigger mites) from encapsulations on hosts and stored
the samples in vials of 70% ethanol until they could be cleared in lactophenol,
slide-mounted in Hoyer’s medium (Walter and Krantz 2009), and identified using
appropriate guides (Brennan and Goff 1977, Hyland 1956, Loomis 1956). To
collect endoparasites, we made a mid-ventral incision from mouth to cloaca, removed
the gastrointestinal tract and other organs, and placed them in a Petri dish
containing 0.6% saline for study under a stereomicroscope. We examined the gall
bladder for myxozoans following methods of McAllister and Trauth (1995). We
used standard histological techniques to prepare tissues for light microscopy following
Presnell and Schreibman (1997). We dehydrated the tissues in a graded
series of increasing ethanol solutions (70–100%), cleared them with xylene, and
infiltrated and embedded in paraffin wax all samples. We trimmed each paraffin
tissue block and used a rotary microtome to sagittally section the samples into
8-μm-thick ribbons. We affixed the ribbons to microscope slides using Haupt’s
adhesive on a slide warmer while floating them on a 2% neutral-buffered formalin
solution. We stained the slides using Harris hematoxylin followed by counterstaining
with eosin (H & E), and applied coverslips using Permount® (Fisher Scientific)
mounting medium. We used a Nikon Eclipse 600 epifluorescent light microscope
with a Nikon DXM 1200C digital camera (Nikon Instruments Inc., Melville, NY)
for photomicroscopy and used the same camera mounted on a Nikon SM2800
stereomicroscope for macrophotography. We used hot tap water to kill trematode
specimens, which we then fixed in 70% DNA-grade ethanol, stained with acetocarmine,
and mounted samples in Canada balsam. We fixed nematodes in hot 70%
DNA-grade ethanol and placed each sample on a glass slide in a drop of undiluted
glycerol for identification. We deposited voucher specimens of ectoparasites in the
general ectoparasite collection in the Department of Biology at Georgia Southern
University, Statesboro, GA (accession nos. L 3683–84) and deposited voucher
specimens of helminths in the Harold W. Manter Laboratory of Parasitology
(HWML), University of Nebraska, Lincoln, NE. We deposited host vouchers in the
Arkansas State University Museum, Herpetological Collection (ASUMZ), Jonesboro,
AR. We provide prevalence, mean intensity, and range of infection (Bush et
al. 1997). Scientific names of amphibians follow Frost (2014).
Results and Discussion
Fourteen (78%) Southern Toads were infected or infested with parasites as
follows: 2 (11%) with Megalodiscus temperatus (Stafford) Harwood (HWML
64779), 1 (6%) with tetrathyridia of Mesocestoides sp. (HWML 75381), 3 (17%)
with Cylindrotaenia americana Jewell (retained in C.T. McAllister’s personal
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C.T. McAllister, C.R. Bursey, M.B. Connior, S.E. Trauth, and L.A. Durden
2015 Vol. 14, No. 4
collection), 9 (50%) with Cosmocercoides variabilis (Harwood) Travassos
(HWML 64780), 10 (55%) with Oswaldocruzia pipiens (Travassos) (HWML
64781), 2 (11%) with 3rd-stage larval Physaloptera sp. (HWML 64782), and 2
(11%) with larval Hannemania hegeneri Hyland (a chigger mite). We observed
multiple infections/infestations of helminths and/or arthropods in 7 (39%) of
these hosts and documented several new host and geographic-distribution records.
We found no myxozoans in the gall bladder.
We observed trematodes of Megalodiscus temperatus in the rectum of Southern
Toad (2 host-toads with 1 trematode each). This organism has been reported from
a variety of anurans, including 3 bufonids, A. americanus americanus Holbrook
(American Toad) from Iowa and ON, Canada, A. fowleri Hinkley (Fowler’s Toad)
from Virginia, and A. woodhousii Girard (Woodhouse’s Toad) from Nebraska
(Bolek and Janovy 2008, Campbell 1968, Stafford 1905, Ulmer 1970). Megalodiscus
temperatus has also been found in caudate amphibians and anurans from
Florida (Loftin 1960, Manter 1938, Parker 1941). Megalodiscus temperatus eggs
are embryonated when laid, and miracidia hatch and penetrate snails of 3 species
of Planorbella (= Helosoma) in which they develop into sporocysts. Cercariae develop
into daughter rediae in the snail hepatopancreas and after leaving the snail,
they encyst as metacercaria in the skin of metamorphosed anurans. Frogs become
infected when they ingest tadpoles or pieces of shed skin, and the adult worm develops
in the rectum. Tadpoles become infected when they ingest the Megalodiscus
metacercariae, which encyst on the bottom of the waterbody. In tadpoles that successfully
undergo metamorphosis into juvenile frogs, M. temperatus flukes migrate
up to the stomach and then back to the rectum (Bolek and Janovy 2008, Krull and
Price 1932). Our observations represent a new host record for M. temperatus.
The abundant Mesocestoides sp. tetrathyridia that we observed in the liver of
Southern Toads possessed characteristic individual features of the non-proliferative
type with a single invaginated scolex, a generally deep invagination canal, a prominent
single excretory pore at the end opposite the scolex, and a solid hindbody
(Fig. 1; also see Conn et al. 2002). Adult Mesocestoides tapeworms are cosmopolitan
parasites of placental mammals, birds, and rarely humans (Padgett et al. 2012).
The tetrathyridial stage is often found in the body cavity and encapsulated in tissues
of vertebrate 2nd-intermediate hosts such as amphibians, reptiles, and rodents.
Recently, McAllister et al. (2014b) provided a summary of North American anuran
hosts of Mesocestoides sp. that included the following bufonid hosts: American
Toad, Anaxyrus americanus charlesmithi (Bragg) (Dwarf American Toad), A. cognatus
Say (Great Plains Toad), Fowler’s Toad, A. houstonensis Sanders (Houston
Toad), and Incilius nebulifer (Girard) (Coastal-Plain Toad). To date, this tapeworm
has been reported in amphibians from 11 (22%) states including Arkansas, California,
Iowa, Kansas, Michigan, Nebraska, New York, Oklahoma, South Dakota,
Texas, and Wisconsin. We document for the first time, Mesocestoides sp. in Southern
Toad and in Florida.
We collected a total of 19 (mean ± 1SD = 6.3 ± 7.5, range = 2–15) Cylindrotaenia
americana from the small intestine of Southern Toad. This nematotaeniid
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2015 Vol. 14, No. 4
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tapeworm was originally reported from Southern Toad (as Bufo lentiginosus
Shaw) from an unknown locality (Jewell 1916). Cylindrotaenia americana has
a large geographic range in the US as well as in Canada, the Caribbean, Central
America, Mexico, and South America (see McAllister et al. 2013) and has been
previously reported from Florida in Scincella lateralis (Say in James) (Ground
Skink; Brooks 1972).
We found 23 Cosmocercoides variabilis nematodes (8 males, 15 females, 4.0 ±
3.6, 1–8) in the lower intestinal tract of Southern Toad. Cosmocercoides variabilis
is a very common intestinal parasite of North American amphibians and reptiles
(see recent summation by Bursey et al. 2012). In Florida, C. variabilis has been
reported from Southern Toad (Walton 1940) and Oak Toad (Hamilton 1955).
This nematode has been previously reported from several other states including,
Arizona, Arkansas, California, Idaho, Illinois, Iowa, Louisiana, Massachusetts,
Michigan, Nebraska, New York, North Carolina, North Dakota, Ohio, Oklahoma,
Oregon, South Dakota, Texas, Utah, Virginia, Washington, West Virginia, and
Wisconsin; the overall distribution also includes Alberta, British Columbia, New
Brunswick, and Quebec, Canada; and Baja California Norte, Mexico; Costa Rica;
and Panama (see Bursey et al. 2012, Connior et al. 2015). We report C. variabilis
in Southern Toad for the second time.
Figure 1. Mesocestoides sp. tetrathyridia
from liver (near the
kidneys) of Southern Toad showing
solid hindbody (H) below the
scolex (S).
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2015 Vol. 14, No. 4
We found 6 third-stage larval Physaloptera sp. (1 in 1 host, 5 in another host)
in Southern Toad stomachs. They are common nematodes of many other anurans,
including Oak Toad in Florida (Goldberg and Bursey 1996) as well as other bufonids
in Arizona, California, New Mexico, North Carolina, Ohio, Oklahoma, Texas,
Utah, and Virginia (see summary by Goldberg et al. 2009). Species assigned to
Physaloptera reach maturity in reptiles, birds, and mammals; they require an insect
intermediate host, and infection can be acquired from ingesting insects containing
infective larvae (Anderson 2000). Larvae ingested by possible paratenic hosts
generally attach to the gastric mucosa and can persist for varying periods of time;
alternately, infective larvae have been found encapsulated in the stomach wall of
various paratenic hosts (Anderson 2000). We found the larva in the stomach lumen;
thus, it is not possible to determine if Southern Toad represents a paratenic host and
the unencysted larvae would be infective to the next host in the life cycle. This is
the first report of larval Physaloptera in Southern Toad.
We recovered 29 Oswaldocruzia pipiens Walton strongylid nematodes (9 males,
20 females, 5.0 ± 4.8, 1–13) from the small intestine of Southern Toad. The species
has been previously reported from various anurans from North America, including
American Toad, Dwarf American Toad, Anaxyrus boreas (Baird & Girard) (Western
Toad), Great Plains Toad, Fowler’s Toad, A. hemiophrys Cope (Canadian Toad),
Houston Toad, A. punctatus (Baird & Girard) (Red-spotted Toad), A. retiformis
(Sanders & Smith) (Sonoran Green Toad), and Woodhouse’s Toad from Arizona,
Arkansas, California, Colorado, Georgia, Massachusetts, Michigan, North Carolina,
Ohio, Oklahoma, Tennessee, Texas, Virginia, West Virginia, and Wisconsin,
and Alberta and Ontario, Canada (see McAllister et al. 2014a). Harwood (1932)
previously reported O. pipiens in Southern Toad from Texas; however, this host
was misidentified and was actually the Houston Toad (see Thomas et al. 1984).
Therefore, this is the first time O. pipiens has been reported from Southern Toad
and Florida.
Hannemania hegeneri was originally described from Lithobates sphenocephalus
utricularius Cope (Southern Leopard Frog) from Sarasota, FL (Hyland 1956).
Loomis (1956) recorded this chigger mite from various amphibians in Arkansas,
Kansas, Missouri, Oklahoma, Nebraska, and Texas under the synonym Hannemania
multifemorala. Although he recorded it from Bufo terrestris in Arkansas, that
host (currently treated as Anaxyrus terrestris [Southern Toad]) is not known to occur
in Arkansas; thus, the host identification must have been an error. Crossley and
Proctor (1971) also recorded H. hegeneri from Southern Leopard Frog (as Rana
pipiens) and Acris crepitans (Baird) (Northern Cricket Frog) in Georgia. Walters
et al. (2011) listed H. hegeneri and H. multifemorala as separate species, recording
H. hegeneri from amphibians in Florida and Georgia and H. multifemorala from
amphibians in Alaska, Arkansas, Kansas, Missouri, Nebraska, Oklahoma, and Texas.
We question the Alaska report because of its distance from the other records and
wonder if this was an unintentional repeat of the Arkansas records because states
were listed by their 2-letter abbreviations by Walters et al. (2011) with both AK and
AR and NB and NE listed (NE is the correct abbreviation for Nebraska). Crossley
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(1960) described the nymph of H. hegeneri based on specimens from Nebraska and
Texas, and Hyland (1961) described the life cycle based on specimens reared from
Sarasota, FL. As with other members of the genus, unfed larvae of H. hegeneri penetrate
the connective tissue of the host dermis where a localized host-tissue reaction
forms an envelope or cyst around the chigger mite (Hyland 1961). Engorgement of
H. hegeneri larvae is prolonged, taking 2–3 wk; engorged larvae may remain inside
the host cyst for an additional period of time so that the total on-host phase can be
as long as 6 mo (Hyland 1961).
Currently, the helminth and arthropod parasite list for Southern Toad contains 2
trematode species, 3 cestode species, 5 nematode species, and 1 arthropod (Table 1).
Further surveys on Southern Toad from other parts of its range (including disjunct
populations) may, in time, increase the number of known parasites; however, our
survey supports Aho’s (1990) suggestion that like most amphibians, Southern Toad
harbors a depauperate noninteractive helminth community.
Acknowledgments
The Florida Fish and Wildlife Conservation Commission provided a scientific collecting
permit (# LSSC 13-00033) to M.B. Connior. We thank Dr. S. L. Gardner (HWML) for expert
curatorial assistance.
Table 1. Summary of helminth and arthropod parasites reported from Southern Toad. Prevalence =
number infected/number examined (percent).
Helminth Locality Prevalence Reference
Trematoda
Megalodiscus temperatus1 Florida 2/18 (11%) This report
Reniferinae (cercaria)2 Florida Not given Sears et al. (2012)
Cestoidea
Cylindrotaenia americana Not given Not given Jewell (1916)
Florida 3/18 (17%) This report
Distoichometra bufonis Dickey Georgia Not given Dickey (1921)
Mesocestoides sp.1, 3 Florida 1/18 (6%) This report
Nematoda
Cosmocercoides variabilis Florida Not given Walton (1940)
Florida 9/18 (50%) This report
Gyrinicola batrachiensis4 (Walton) Florida 1/1 (100%) Pryor and Greiner (2004)
Oswaldocruzia pipiens1, 3 Florida 10/18 (55%) This report
Physaloptera sp.1 Florida 2/18 (11%) This report
Rhabdias americanus Baker Florida Not given Walton (1938)
Arthropoda
Hannemania hegeneri1 Florida 2/18 (11%) This report
1New host record.
2Experimental infection.
3New distribution record.
4Tadpoles only.
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