Northeastern Naturalist Vol. 22, No. 1 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 213 2015 NORTHEASTERN NATURALIST 22(1):213–222 Current Status and Distribution of Etheostoma exile (Iowa Darter) and Erimyzon sucetta (Lake Chubsucker) in Ohio Justin S. Baker1,2,*, Brian J. Zimmerman1, and Marymegan Daly1 Abstract - A rich fish assemblage that once thrived in the bays of western Lake Erie, and Ohio’s glacial pothole lakes, wetlands, and clear prairie streams has changed over the past century. Three species have been extirpated from many of their historic localities, with surviving members reduced to relict populations. Recognizing that conservation efforts for these sensitive species rely on accurate occurrence records, we present results from 2011–2012 surveys of Ohio’s glacial lakes and associated habitats for 2 native members of this fish assemblage, Etheostoma exile (Iowa Darter) and Erimyzon sucetta (Lake Chubsucker). We found previously undocumented populations of Lake Chubsuckers in 4 glacial lakes and a previously undocumented population of Iowa Darters; however, comparing our results to surveys completed in the 1980s indicates that both species have been extirpated from many localities. A variety of factors including intensive agricultural practices, urban development, and exotic species are likely the cause of their d ecline. Introduction In Ohio, nearly 90% of historical wetlands have been drained (Dahl 1990, Fretwell et al. 1996). This includes a massive wetland, the Great Black Swamp, which encompassed 3885 km2 in northwestern Ohio, extending from Lake Erie to New Haven, IN (Kaatz 1955, Ohio Department of Natural Resources 1988). Widespread drainage and other alterations have resulted in the patchy distribution of native fishes in isolated marshes and glacial pothole lakes. Historical records indicate that species native to these habitats have been declining. Notropis anogenus Formes (Pugnose Shiner), Notropis heterodon (Cope) (Blackchin Shiner), and Notropis heterolepis Eigenmann and Eigenmann (Blacknose Shiner) have been extirpated from Ohio, and others are currently listed as endangered or threatened (ODNR 2012, Trautman 1981). In this study, we assess the status in Ohio of 2 members of this listed fish assemblage: Etheostoma exile (Girard) (Iowa Darter) and Erimyzon sucetta (Lacepède) (Lake Chubsucker). The Iowa Darter has the most northern geographic range of any darter. It is found throughout the interior of southern Canada, from Alberta to eastern Ontario, and the northern United States (Page and Burr 2011). This species typically inhabits clear glacial lakes, springs, and small streams that have dense beds of submerged aquatic vegetation and a bottom of sand, peat, or organic debris (Trautman 1981). The habits and behaviors of this species make it sensitive to increased turbidity and the destruction of submerged aquatic vegetation. Trautman (1981) noted that Iowa 1Museum of Biological Diversity, The Ohio State University, Columbus, OH 43212. 2Current address - Amec Foster Wheeler E & I, Ballwin, MO 63011. *Corresponding author - justin.baker@amecfw.com. Manuscript Editor: Thomas J. Maier Northeastern Naturalist 214 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 Vol. 22, No. 1 Darter has been greatly reduced in numbers in Ohio since 1930, with many historical populations extirpated. The Lake Chubsucker can be found along the Atlantic Slope from Virginia to southern Florida, along Gulf Slope drainages to central Texas, within the central Mississippi River valley, in eastern Midwest drainages, and in tributaries of the Great Lakes (Page and Burr 2011). Across its range, the Lake Chubsucker occupies glacial lakes, marshes, ditches, and the calm parts of streams having dense beds of submerged aquatic vegetation with bottoms of sand or muck (Wall and Gilbert 1980). Trautman (1981) suggested that populations of Lake Chubsuckers are highly intolerant of turbidity and siltation, although Jenkins and Burkhead (1993) suggested that siltation might not be a general limiting factor. Before 1900, the Lake Chubsucker was present in marshes along Lake Erie and possibly even the Ohio River before dam construction (Osburn 1901, Trautman 1981). Other populations that were once healthy, such as those present in East Harbor (Lake Erie) and Buckeye Lake (Licking County), began declining after 1930 and were extirpated by 1950 (Trautman 1981). The purpose of the present study was to update distribution records for Iowa Darters and Lake Chubsuckers in the State of Ohio, the seventh most densely populated state in the US (USCB 2014). Such an effort is necessary for any management and conservation of these sensitive species. To assess the decline in these species over the past 30 years, we compared our results with those of surveys of Ohio’s glacial lakes completed in the fall of 1980 and the springs of 1981 and 1985 (Phinney and Rice 1984; D. Rice, Division of Natural Areas and Preserves, Columbus, OH, 1985 unpubl. data). Methods We sampled 66 localities between April 2011 and June 2012 (Fig. 1). These included glacial lakes, marshes, bogs, fens, remnants of the previous Ohio–Erie canal system, and small streams. We selected sampling locations based on surveys by Phinney and Rice (1984; D. Rice, 1985 unpubl. data) and previous records from the Ohio Environmental Protection Agency (OEPA; D. Mishne, Division of Surface Water, Groveport, OH, 1979 to 2010 unpubl. data). A total of 49 localities for the Iowa Darter and the Lake Chubsucker were revisited; we did not revisit those that Phinney and Rice (1984) judged as extirpated (e.g., Buckeye Lake, Licking County; Lake Erie, Ottawa County). Locations that had not been sampled previously were also investigated and were selected based on their proximity to nearby locations with records (e.g., Snow Lake, Geauga County). To enhance visibility, we completed surveys during March to early August, prior to the full development of emergent vegetation. We chose collecting methods based on the species targeted, habitat, and accessibility of the sampling locality. Collections of Iowa Darters were made by seining shoreline habitats using a 1.2 m × 2.4 m, 6.35-mm-mesh seine with a chain-weighted lead line. To sample Lake Chubsuckers, we used a flat-bottom jon boat, 4.3 m in length with a shallow draft, equipped with a 5.0 generatorNortheastern Naturalist Vol. 22, No. 1 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 215 powered pulsator (GPP) electrofisher unit (Smith-Root, Vancouver, WA). Glacial lakes that were inaccessible with the electrofishing boat were sight-surveyed using kayaks in early June. At this time, small, young Lake Chubsuckers could be observed schooling in shallow water along with young-of-year Notemigonus crysoleucas (Golden Shiners). We distinguished young Lake Chubsuckers from young Golden Shiners by the distinct black band that extends from the snout to tail and the black pigment on the anterior rays of the dorsal fin characteristic of the Lake Chubsuckers. Voucher specimens of fish observed via kayak were collected using dip-nets. All voucher specimens were first euthanized with tricaine methane sulfonate (TMS, MS-222) and then preserved in 10% formalin in accordance with IACUC-approved protocol #2011A00000050 and deposited at the Ohio State University Museum of Biological Diversity. To test for changes in the range (i.e., the number of sites occupied) of each species, we compared presence–absence data from 1980–1989 and 2011–2012 at the 49 sites visited during both periods using McNemar’s test (McNemar 1947, Sokal and Rohlf 1995). This test is based on the null hypothesis that the number of sites changing status from present to absent is equal to the number of sites changing from absent to present (i.e., no change between periods in the number of sites occupied by each species). Figure 1. Map showing current and historical distribution of Etheostoma exile (Iowa Darter) and Erimyzon sucetta (Lake Chubsucker) in Ohio. Asterisks denote records before 1980, open circles indicate records from the 1980s, light gray triangles indicate records from 1990–2010 (see text), and solid gray circles indicate localities where specimens were obtained during the current study. Insets show the geographic range of each species, taken from Page and Burr (2011). Northeastern Naturalist 216 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 Vol. 22, No. 1 Results and Discussion A total of 232 Iowa Darters were collected from 12 localities distributed across 7 counties (Fig. 1, Appendix 1). Four of the 12 localities were represented by 4 or fewer individuals: Nettle Lake, Stewart’s Pond, Silver Lake (Logan County), and Doke Lake (Appendix 1). Despite previous surveys (e.g., D. Rice, 1985 unpubl. data), the three Iowa Darters collected from Stewart’s Pond (Portage County) were the first reported from this location. The largest population of Iowa Darters we encountered was from Round Lake (Ashland County), with 32 individuals caught per hour. Despite multiple attempts, we failed to catch any Iowa Darters in Potter Creek (Portage County), a locality first sampled successfully by OEPA in 2000. Seventeen locations at which Iowa Darters had been recorded in the 1980s surveys by Phinney and Rice (1984) yielded no specimens during our surveys (T able 1). A total of 236 Lake Chubsuckers were collected from 16 localities distributed across 8 counties (Fig. 1, Appendix 2). Only 1 of these (Turkeyfoot Lake, Summit County) also harbored a population of Iowa Darters. Surveys of 4 glacial lakes resulted in new records (Snow and Aquilla Lakes, Geauga County; Lake Kelso, Portage County; Lemen Lake, Logan County). Additionally, a single individual was collected from the Clear Fork Saint Joseph River (Williams County). The collection of 7 individuals from Turkeyfoot Lake was the first record of Lake Chubsucker from this locality since 1926. Our survey of 20 locations known to support populations of Lake Chubsuckers during the 1980s suggests that only 7 still maintain populations (Table 1). Populations of Iowa Darters and Lake Chubsuckers are in decline in Ohio. The range of Iowa Darters has contracted sharply from 57% of sites in 1980–1989 to only 24% of sites in 2011–2012 when comparing 49 sites sampled during both periods (P = 0.0017; Table 1). This change represents a 57% decrease in the number of sites occupied by Iowa Darters since the 1980s. Moreover, one-third of those sites currently occupied were represented by 4 or fewer individuals in our collections. The pattern of decline is less clear with Lake Chubsuckers. When compared with glacial lake surveys completed during the 1980s, Lake Chubsuckers showed a 45% decrease in the number of lakes occupied (from 40% of sites in 1980–1989 to 22% of sites in 2011–2012; P = 0.0859; Table 1). Collection records from Rice and Phinney (D. Rice, 1993 unpubl. data), and OEPA (D. Mishna, 1995 unpubl. data) in the 1990s, however, indicate a substantial population in the Symmes Creek drainage of southern Ohio in Gallia and Jackson counties (131 individuals caught from 10 Table 1. Summary of changes in the distribution of Etheostoma exile (Iowa Darter) and Erimyzon sucetta (Lake Chubsucker) between 1980–1989 and 2011–2012. Numbers in parentheses indicate the number of occurrences at 49 sites visited during both periods. * indicates statistical significance (P < 0.05). Number of occurrences Changes in range Species 1980–1989 2011–2012 Historical sites between periods Etheostoma exile 32 (28) 12 (12) 11 -57%* Erimyzon sucetta 21 (20) 16 (11) 7 -45%* Northeastern Naturalist Vol. 22, No. 1 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 217 localities; Fig. 1). Records from the West Branch Cuyahoga River (Geauga County) also indicate another potentially large connected population of Lake Chubsuckers in northeastern Ohio (D. Mishna, 1993 unpubl. data). Additionally, we report 4 new records of Lake Chubsuckers in separate glacial lakes, along with a population in Turkeyfoot Lake that was thought to have been extirpated. Furthermore, our surveys and those of ODNR (R. Moss, Ohio Department of Natural Resources, Columbus, OH, 2001 unpubl. data) indicate another potentially large population in the Killbuck Marsh Wildlife Area (Holmes and Wayne counties; Fig. 1). Thus, while many glacial lake populations have disappeared since the 1980s, other populations occupying separate glacial lakes, marshes, and streams have been discovered (Sanders et al. 1999, this study). The decline over the past 30 years in number of glacial lake populations of both Iowa Darters and Lake Chubsuckers are likely the result of a variety of factors involving agricultural land-use practices, nearby urban development, and exotic species. Conclusions and conservation implications The pattern of decline in the number of populations for two Ohio-native species inhabiting glacial lakes and wetlands are not identical. The Lake Chubsucker seems less impacted than the Iowa Darter, having generally larger populations and being recovered in places from which it was previously thought to have been extirpated. This difference may reflect broader physiological or historical factors, e.g., that Ohio populations of Iowa Darters are towards the southern limit of their range and may fare less well than those of Lake Chubsuckers, for which Ohio is near the middle of this species’ range. Alternatively, the difference may relate to sampling strategy, as we were able to collect Lake Chubsuckers using electrofishing and sight surveys, whereas Iowa Darters were collected via seining. Differences in overall number of sites and populations withstanding, many of the same factors likely contribute to the century-long decline in these species in Ohio. Mitigation of these factors will likely benefit both species, although the timescale and magnitude of recovery may differ between Iowa Darters and Lake Chubsuckers. Increased communication with private landowners concerning better land-use practices and lake management may help resolve several of these issues (Carpenter and Lathrop 1999). In other cases, control of nonnative species (e.g., Cyprinus carpio L. [Common Carp]) may be necessary to reduce turbidity and improve water quality and the growth of submerged aquatic vegetation (Pinto et al. 2005). Further management and conservation strategies could also involve the captive propagation and/or translocation of species. For example, several of the glacial lakes with records of Iowa Darters that we surveyed during this study contained suitable habitat for possible reintroductions (e.g., Crystal Lakes, Summit and Portage counties). Other glacial lakes such as Baker Lake (Champaign County) and Stewart’s Pond (Portage County) have a high diversity of submerged aquatic plants and may provide potential sites to introduce Lake Chubsuckers. Similar conservation strategies could also be developed for other associated species that once occurred in Ohio such as Notropis heterodon (Blackchin Shiners) and Northeastern Naturalist 218 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 Vol. 22, No. 1 N. heterolepis (Blacknose Shiners), with careful consideration given to the genetic diversity of source populations used for reintroduction (Ozer and Ashley 2013), as well as genetic differentiation among populations, which may indicate adaptation to local conditions (McKay et al. 2005). Restoration of these sensitive species back into Ohio’s glacial lake assemblage will require knowledge of current population trends and potential factors associated with their decline, in addition to carefully planned captive propagation and/or translocation projects. Acknowledgments This work was supported by a State Wildlife Grant from the US Fish and Wildlife Service through the Ohio Biodiversity Conservation Partnership with the Ohio Division of Wildlife and the Ohio State University. We thank B. Alsdorf, S. Chambers, M. Delavega, K. Holland, M. Kibbey, E. Moran, M. Sarver, E. Simmons, N. Tessler, and D. Zimmerman for their assistance with field work. We also thank the many private landowners and city municipalities that allowed us access to glacial lakes on their property, in addition to two anonymous Reviewers and Manuscript Editor, Thomas J. Maier. Literature Cited Carpenter, S.R., and R.C. Lathrop. 1999. Lake restoration: Capabilities and needs. Hydrobiologia 396:19–28. Dahl, T.E. 1990. Wetlands: Losses in the United States, 1780s to 1980s. US Fish and Wildlife Service Report to Congress. Washington, DC. 13 pp. Fretwell, J.D., J.S. Williams, and P.J. Redman. 1996. National water summary on wetland resources. Water-supply paper 2425. US Geological Survey, Washington, DC. 431 pp. Jenkins, R.E., and N.M. Burkhead. 1993. Freshwater Fishes of Virginia. American Fisheries Society, Bethesda, MD. 1079 pp. Kaatz, M.R. 1955. The black swamp: A study in historical geography. Annals of the Association of American Geographers 45(1):1–35. McKay, J.K., C.E. Christian, S. Harrison, and K.J. Rice. 2005. “How local is local?” A review of practical and conceptual issues in the genetics of restoration. Restoration Ecology 13(3):432–440. McNemar, Q. 1947. Note on the sampling error of the difference between correlated proportions or percentages. Psychometrika 12(2):153–157. Ohio Department of Natural Resources (ODNR). 1988. Ohio wetlands priority conservation plan. Office of Outdoor Recreational Services, Columbus, OH. 67 pp. ODNR. 2012. Wildlife that are considered to be endangered, threatened, species of concern, special interest, extirpated, or extinct in Ohio. Division of Wildlife, Columbus, OH. 10 pp. Osburn, R.C. 1901. The fishes of Ohio. Ohio Academy of Science Special Paper, No. 4. Columbus, OH. 105 pp. Ozer, F., and M.V. Ashley. 2013. Genetic evaluation of remnant and translocated shiners, Notropis heterodon and Notropis heterolepis. Journal of Fish Biology 82:1281–1296. Page, L.M., and B.M. Burr. 2011. Peterson field Guide to Freshwater Fishes of North America North of Mexico. Houghton Mifflin Harcourt, New York, NY. 663 pp. Phinney, G., and D.L. Rice. 1984. New records of the Iowa Darter, Etheostoma exile, (Percidae) in Ohio. Ohio Journal of Science 84(1):67–70. Northeastern Naturalist Vol. 22, No. 1 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 219 Pinto, L., N. Chandrasena, J. Pera, P. Hawkins, D. Eccles, and R. Sim. 2005. Managing invasive carp (Cyprinus carpio L.) for habitat enhancement at Botany Wetlands, Australia. Aquatic Conservation: Marine and Freshwater Ecosystems 15:447–4 62. Sanders, R.E., C. Staudt, D. Mishne, M. Smith, E.T. Rankin, et al. 1999. The frequency of occurrence and relative abundance of Ohio stream fishes: 1979 through 1995. Ohio Biological Survey Notes 2:53–62. Sokal, R.R., and F.J. Rohlf. 1995. Biometry: the Principles and Practice of Statistics in Biological Research. 3rd Edition. W.H. Freeman, New York, NY. 880 pp. Trautman, M.B. 1981. The Fishes of Ohio. Ohio State University Press, Columbus, OH. 782 pp. United States Census Bureau (USCB). 2014. Estimates for 01 July 2014 for the 50 States. Available online at http://www.census.gov/popest/data/index.html. Accessed 29 August 2014. Wall, B.R., Jr., and C.R. Gilbert. 1980. Erimyzon sucetta (Lacepede), Lake Chubsucker. Pp. 399, In D.S. Lee, et al. (Eds.). Atlas of North American Freshwater Fishes. North Carolina State Museum of Natural History, Raleigh, NC. 854 pp. Northeastern Naturalist 220 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 Vol. 22, No. 1 Appendix 1. Collection data for Etheostoma exile (Iowa Darter) from the present study as well as collections from the 1980s. Number Collection Catalogue # caught County Locality Latitude Longitude date Collection data from the present study OSUM 112721 50 Ashland Round Lake 40°40'13"N 82°8'34"W 4/5/12 OSUM 111421 20 Clark Crystal Lakes 39°53'16"N 84°1'26"W 6/2/11 OSUM 111422 10 Clark Crystal Lakes 39°53'39"N 84°1'27"W 6/2/11 OSUM 111720 4 Logan Doke Lake 40°18'37"N 83°47'53"W 4/8/11 OSUM 111385 25 Logan East Twin Lake 40°19'13"N 83°47'33"W 4/29/11 OSUM 111709 3 Logan Silver Lake 40°1'14"N 83°48'45"W 8/9/11 OSUM 111416 30 Miami Silver Lake 39°57'18"N 84°3'9"W 6/2/11 OSUM 112737 3 Portage Stewart’s Pond 41°11'30"N 81°21'30"W 4/23/12 OSUM 112756 25 Portage East Twin Lake 41°11'35"N 81°20'3"W 4/25/12 OSUM 112750 20 Portage West Twin Lake 41°11'41"N 81°20'21"W 4/25/12 OSUM 112726 35 Summit Turkeyfoot Lake 40°58'10"N 81°32'15"W 4/12/12 OSUM 111490 1 Williams Nettle Lake 41°41'2"N 84°43'38"W 8/2/11 Collection data from the 1980s OSUM 48623 10+ Ashland Round Lake 40°40'13"N 82°8'34"W 10/22/80 OSUM 48614 10+, Clark Crystal Lakes, 39°53'16"N 84°1'26"W 11/10/80, southernmost lake N/A 20 5/6/85 N/A 2 Clark Crystal Lakes, 39°53'39"N 84°1'27"W 5/8/85 middle lake N/A 2 Clark Crystal Lakes, 39°53'43"N 84°1'30"W 5/8/85 northernmost lake OSUM 48627 4+ Geauga Geauga Lake 41°20'54"N 81°22'27"W 11/7/80 OSUM 48694 3 Geauga Punderson Lake 41°27'18"N 81°12'27"W 7/1/80 OSUM 48630 3 Holmes Long/Bonnett Lake 40°39'60"N 82°8'10"W 10/22/80 OSUM 48624 10+, Logan Doke Lake 40°18'37"N 83°47'53"W 10/2/80, N/A 2 4/26/85 OSUM 48621 3, Logan East Twin Lake 40°19'13"N 83°47'33"W 8/27/80, N/A 10+ 9/30/80 OSUM 48618 10+ Logan West Twin Lake 40°19'21"N 83°47'41"W 9/30/80 OSUM 48612 6+ Logan Silver Lake 40°21'14"N 83°48'45"W 10/6/80 OSUM 74040 4 Logan Lemen Lake 40°17'15"N 83°49'15"W 5/28/85 OSUM 74029 4 Logan Braden Lake 40°17'15"N 83°49'6"W 4/15/85 N/A 5, Logan Newell Lake 40°16'60"N 83°48'31"W 9/30/80, OSUM 74026 12 4/15/85 OSUM 48622, 4+, Logan McMillen Lake 40°19'4"N 83°49'45"W 10/27/80, OSUM 74261 32 4/23/85 OSUM 48620 1, Miami Silver Lake 39°57'18"N 84°3'9"W 9/8/80, N/A 3 5/6/85 OSUM 48625 1 Portage Crystal Lake 41°7'52"N 81°14'11"W 12/8/80 OSUM 48626 10+ Portage East Twin Lake 41°11'35"N 81°20'3"W 12/9/80 N/A 5+ Portage West Twin Lake 41°11'41"N 81°20'21"W 12/9/80 OSUM 48973 6, Portage Pippen Lake 41°10'40"N 81°18'42"W 3/25/81, N/A 2 10/21/87 OSUM 48613 2 Richland Sites Lake 40°46'58"N 82°23'28"W 11/20/80 OSUM 48628, 2, Stark Meyers Lake 40°48'60"N 81°25'4"W 12/2/80, OSUM 55222 35 5/25/83 OSUM 48629 2, Summit Crystal Lake 41°9'48"N 81°27'57"W 11/20/80, N/A 10+, 3/25/81, N/A 5 10/22/87 Northeastern Naturalist Vol. 22, No. 1 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 221 Appendix 2. Collection data for Erimyzon sucetta (Lake Chubsucker)from the present study as well as collections from the 1980s. Number Collection Catalogue # caught County Locality Latitude Longitude date Collection data from the present study OSUM 112761 2 Geauga Aquilla Lake 41°32'52"N 81°10'6"W 5/1/12 OSUM 112780 3 Geauga Snow Lake 41°25'34"N 81°10'33"W 5/24/12 OSUM 114106 2 Geauga West Branch 41°28'17"N 81°10'42"W 6/25/12 Cuyahoga River OSUM 112767 2 Geauga Lake Kelso 41°26'40"N 81°10'30"W 5/2/12 OSUM 112711 15 Holmes Lower Killbuck 40°28'49"N 81°59'20"W 4/3/12 Marsh OSUM 111714 4 Holmes Marsh attached to 40°28'53"N 81°59'45"W 6/26/11 Wolf Creek OSUM 114024 10 Jackson Jackson Lake 38°54'26"N 82°35'43"W 5/31/12 OSUM 111717, 16, Logan Braden Lake 40°17'15"N 83°49'6"W 4/19/11, OSUM 111715 7 7/26/11 OSUM 111474 30 Logan Lemen Lake 40°17'15"N 83°49'15"W 7/26/11 OSUM 111475 6 Logan Newell Lake 40°16'60"N 83°48'31"W 7/26/11 OSUM 111435, 20, Logan Rush Creek Lake 40°24'16"N 83°40'54"W 6/7/11, OSUM 111482 15 7/27/11 OSUM 112724 7 Summit Turkeyfoot Lake 40°58'10"N 81°32'15"W 4/12/12 OSUM 112719 1 Wayne Killbuck Marsh 40°42'23"N 81°59'8"W 4/4/12 Wildlife Area OSUM 112714, 4, Wayne Shreve Creek in 40°40'56"N 81°58'17"W 4/4/12, OSUMU 33230 15 Killbuck Marsh 6/21/12 OSUM 111388 1 Williams Clear Fork Saint 41°41'41"N 84°37'29"W 5/4/11 Joseph River OSUM 111397, 1, Williams Mud Lake Bog 41°38'51"N 84°46'2"W 5/4/11, OSUM 111483 100 observed 8/1/11 Collection data from the 1980s OSUM 48665 1 Ashland Round Lake 40°40'13"N 82°8'34"W 10/22/80 OSUM 74266 6 Champaign Brush Lake 40°10'9"N 83°34'49"W 4/24/85 OSUM 74251 3 Champaign Fudger Lake 40°6'10"N 83°32'1"W 4/24/85 OSUM 78477 1 Champaign Glady Creek 40°10'36"N 83°48'3"W 7/31/86 OSUM 74043 1 Geauga Fern Lake 41°26'27"N 81°10'24"W 7/23/85 OSUM 48946 1 Geauga La Due Reservoir 41°24'13"N 81°11'23"W 3/27/81 OSUM 48693, 3, Geauga Punderson Lake 41°27'18"N 81°12'27"W 7/1/80, OSUM 74248 16 5/1/85 OSUM 73228 2 Geauga West Branch 41°27'1"N 81°9'30"W 10/13/88 Cuyahoga River OSUM 74039 2 Jackson Jackson Lake 38°54'26"N 82°35'43"W 6/13/85 N/A 25 Logan Braden Lake 40°17'15"N 83°49'6"W 4/15/85 OSUM 74242 3 Logan Doke Lake 40°18'37"N 83°47'53"W 4/26/85 OSUM 74255 1 Logan McMillen Lake 40°19'4"N 83°49'45"W 4/23/85 N/A 30 Logan Newell Lake 40°16'60"N 83°48'31"W 4/15/85 OSUM 74249 70 Logan Rush Creek Lake 40°24'16"N 83°40'54"W 4/30/85 OSUM 49745 2 Pickaway Circleville Canal 39°35'2"N 82°58'22"W 9/30/81 OSUM 74264 2 Portage Muzzy Lake 41°7'4"N 81°15'6"W 5/2/1985 OSUM 74263 3 Summit Long Lake 41°0'31"N 81°32'32"W 5/3/1985 OSUM 78465 1 Summit North Reservoir 41°0'0"N 81°33'1"W 5/14/84 Northeastern Naturalist 222 J.S. Baker, B.J. Zimmerman, and M. Daly 2015 Vol. 22, No. 1 Number Collection Catalogue # caught County Locality Latitude Longitude date N/A 32 Wayne Shreve Creek in 40°40'56"N 81°58'17"W 8/23/85 Killbuck Marsh OSUM 52275, 3, Williams Mud Lake Bog 41°38'51"N 84°46'2"W 7/21/82, N/A, 5, 5/10/85, N/A, 18, 9/29/89, N/A 7 10/3/89 OSUM 49746, 1, Williams Nettle Lake 41°40'48"N 84°43'40"W 10/1/81, OSUM 73407 1 10/28/87