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Prevalence of Salmonella sp. Isolated from Cryptobranchus alleganiensis alleganiensis in Eastern Tennessee
Bridgid Lammers, David Bemis, Phil Colclough, and Marcy J. Souza

Southeastern Naturalist, Volume 11, Issue 1 (2012): 59–64

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2012 SOUTHEASTERN NATURALIST 11(1):59–64 Prevalence of Salmonella sp. Isolated from Cryptobranchus alleganiensis alleganiensis in Eastern Tennessee Bridgid Lammers1, David Bemis2, Phil Colclough3, and Marcy J. Souza2,* Abstract - Cryptobranchus alleganiensis alleganiensis (Eastern North American Hellbender) were collected from the Little River of the Great Smoky National Park (n = 22) and the Hiwassee River of the Cherokee National Forest (n = 30), TN. The cloaca of each animal was swabbed for Salmonella culture and identification. No Salmonella was identified from any of the samples. The prevalence of Salmonella in Hellbenders may be higher than reported here since feces were not collected and animals were only sampled once. Park visitors should still take precautions to protect themselves from water-borne zoonotic pathogens. Introduction The enteric bacterium Salmonella presents a challenge to public health (Herikstad et al. 2002). Many Salmonella serotypes have been isolated from a variety of reptile and amphibian hosts and have been linked to the occurrence of disease in humans (Burnham et al. 1998, CDC 2009, Clarkson et al. 2010, Lockhart et al. 2008, Mermin et al. 2004, Rosenstein et al. 1965, Srikantiah et al. 2004, Van Meervenne et al. 2009, Woodward et al. 1997). Annually in the United States, approximately 74,000 people are infected with Salmonella via contact with an amphibian or reptile. Historically, amphibians have had a lower prevalence of Salmonella than reptiles (Chambers and Hulse 2006, Pfleger et al. 2003). However, amphibians worldwide have been shown to carry Salmonella at least occasionally (Bartlett et al. 1977, Chambers and Hulse 2006, Everard et al. 1979, Kourany et al. 1970, Pfleger et al. 2003, Sharma et al. 1974, Thomas et al. 2001, Trust et al. 1981). Studies have linked human salmonellosis with amphibian reservoirs (CDC 2009, Clarkson et al. 2010, Mermin et al. 2004, Pfleger et al. 2003, Srikantiah et al. 2004). Although the occurrence of Salmonella infections in humans and domestic animal reservoirs has been monitored, few studies have examined the prevalence of this pathogen in wildlife, and in particular amphibians (Chomel et al. 2007). Even less is known about Salmonella in salamander species. Chambers and Hulse (2006) found that among wild-caught native amphibians, salamanders had a lower prevalence (11%) of Salmonella than did all amphibian species combined (39%). There are no data available on the prevalence of 1University of Tennessee College of Veterinary Medicine, Knoxville, TN, 37996. 2Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, TN 37996. 3Knoxville Zoological Gardens, PO Box 6040, Knoxville, TN 37914. *Corresponding author - 60 Southeastern Naturalist Vol. 11, No. 1 Salmonella in Cryptobranchus alleganiensis alleganiensis (Daudin) (Eastern North American Hellbender). The Eastern North American Hellbender may sit on the top of the food chain in the aquatic ecosystems that it inhabits (Nickerson and Mays 1973). A great diversity of Salmonella has been found in natural ecosystems, especially in rivers, and the cool, well-oxygenated, alkaline streams that Hellbenders are found in may provide an ideal media for the growth of Salmonella (Barzily and Kott 1991, Baudart et al. 2000, Morse and Duncan 1974, Nickerson et al. 2003). It has been demonstrated that direct contact with a shedding animal is not necessary to transfer Salmonella from animal to human, and the pathogen can remain viable in water even after the animal has been removed (Bartlett et al. 1977, Rosenstein 1965, Srikantiah et al. 2004, Van Meervenne et al. 2009). There has been no published research examining the potential contamination of waterways with Salmonella from native amphibian species; this contamination could potentially lead to human salmonellosis in park visitors. This study examined the prevalence of cloacal Salmonella in two distinct populations of Hellbenders in East TN. Materials and Methods Study area Hellbender populations are present in the Little River (LR) of the Great Smoky Mountain National Park (GSMNP), TN and Hiwassee River (HR) of the Cherokee National Forest (CNF), TN. Both rivers are used for many recreational purposes such as boating, fishing, swimming, and sightseeing/camping along the bank. The GSMNP and the CNF have millions of visitors annually, with an unknown portion of these visitors engaging in water-related activities. Study sites River sites were chosen based on accessibility, appropriate habitat of shallow, cool, fast moving waters, and the presence of known Hellbender populations from historical surveys. Sites were surveyed from April to September 2010. Animal collection and sampling Hellbenders were collected by hand in both rivers. Disposable nitrile gloves were worn by investigators and were changed after handling and sampling each animal. Animals were placed in cloth or plastic bags with water for holding. Cloth bags were not re-used until laundered with detergent in hot water; plastic bags were disposed of after one use. A swab (BBL™ Culture- Swab Plus™, Becton Dickinson and Co., Sparks, MD) was inserted through the vent into the cloaca and rotated 3–4 times. The swab was then placed into the sterile transport media and kept cool. Morphometrics (total length [cm], snout-to-vent length [cm], mass [g], and age class) were recorded. Age class (adult, sub-adult, juvenile, larva) determination was based on length, mass, and observer experience. PIT tags were placed in each animal over 40 g 2012 B. Lammers, D. Bemis, P. Colclough, and M.J. Souza 61 for identification, and GPS coordinates were recorded for each animal’s collection site. Bacterial cultures Cloacal swabs underwent a routine bacteriology screen for Salmonella. This procedure included MacConkey’s agar (MAC), Hektoen (HE) agar, and Selenite broth incubated at 35° Celsius. At 24 hours, any colonies seen were recorded; if no growth or only very small colonies were observed, the plates were incubated for an additional 24 hours. If lactose negative colonies were identified, they were tested for oxidase. All oxidase negative colonies were screened with Triple sugar iron (TSI) agar, urea agar, and Motility Indole Ornithine (MIO) agar. If the selenite broth was cloudy at 24 hours, the contents were subcultured to HE agar. If selenite broth appeared clear at 24 hours, it was incubated for 48 hours before subculture. All negative plates were held for 48 hours before discarding. Statistical analyses Prevalence and 95% confidence intervals (CI) were calculated for each of the two Hellbender populations. Odds-ratio with confidence intervals was calculated to determine if significant differences in prevalence between the two populations were present (P < 0.05). Results A total of 52 Hellbenders were sampled from both rivers (LR, n = 22; HR, n = 30). Adults (LR, n = 8; HR, n = 11), sub-adults (LR, n = 0; HR, n = 1), juveniles (LR, n = 5; HR, n = 17) and larvae (LR, n = 9; HR, n = 1) were collected from both rivers. Morphometrics are shown in Table 1. Table 1. Morphometric data of Eastern North American Hellbenders (Cryptobranchus alleganiensis alleganiensis) caught and sampled in two eastern Tennessee rivers, the Little and Hiwassee. Age class, snout-to-vent length (SVL; cm), total length (TL; cm) and mass (g) are reported according to which river animals were collected. SVL mean ± SD TL mean ± SD Mass mean ± SD River Age class (n) (range) (range) (range) Hiwassee Adult (11) 20.2 ± 3.4 30.3 ± 4.4 183.8 ± 78.3 (15.6–26) (23.5–36) (86–330) Sub-adult (1) 19.1 33.0 197 Juvenile (17) 13.8 ± 4.8 21.2 ± 5.9 80.2 ± 62.2 (3.2–22.2) (6.5–31.8) (8–235) Larvae (1) 2.5 4.0 1.0 Little Adult (8) 21.0 ± 5.1 32.2 ± 8.5 206.0 ± 106.5 (12.7–26.7) (19.7–41.3) (36–337) Juvenile (5) 8.8 ± 3.4 13.7 ± 5.0 19.8 ± 14.6 (3.8–12.4) (6.4–19.7) (2–35) Larvae (9) 4.6 ± 1.3 6.7 ± 2.2 3.8 ± 2.5 (3–7.6) (4–11.4) (2–8) 62 Southeastern Naturalist Vol. 11, No. 1 The prevalence of cloacal Salmonella isolated from Hellbenders was 0% [95% CI = 0–6.85] from the Little River and Hiwassee River. Because no Salmonella was isolated from either population, no further statistics were performed. Discussion The lack of cloacal Salmonella isolated from Hellbenders in eastern TN suggests a small risk of transmission to humans in the Little and Hiwassee Rivers. Although the prevalence was zero, various factors could have led to a lower measured level than the true prevalence. Salmonella can be shed intermittently by a carrier animal, and fecal samples are typically collected over the course of 3–5 days for culture and identification. Fecal samples are generally preferred over swabs to isolate Salmonella. Sampling a wild animal over numerous consecutive days is not feasible, and fecal samples are not reliably produced by animals. Animals were only held captive for 10–15 minutes during this study. Therefore, single, cloacal swabs were collected from each animal. The true prevalence of Salmonella in Hellbender populations may be higher than that found in this study. The total number of Hellbenders in either of the rivers is not known, and sampling a larger percentage of the population would certainly increase the power of the study. These animals were being collected as part of another study and were sampled opportunistically for Salmonella. It is difficult to know if the sampled population is representative of the entire population of Hellbenders in each river. There are few studies examining the prevalence of Salmonella in wild amphibians, and none in Hellbenders. Future studies may keep animals captive for longer periods of time in order to collect a fecal sample; the stress associated with extended capture would need to be evaluated. Collecting more animals would increase the likelihood of accurately sampling the entire population. Outbreaks of human salmonellosis associated with amphibians have been reported, and monitoring of Salmonella in various amphibian species is warranted. Additionally, humans coming in contact with amphibians or water potentially contaminated by amphibians should be warned to wash themselves after contact and to not drink untreated water. These precautions will reduce the risk of contracting Salmonella, as well as other zoonotic pathogens found in untreated water such as Giardia and Cryptosporidium. Acknowledgments This research was approved by the University of Tennessee IACUC. We would like to thank Arnold Saxton, the volunteers that aided in collection of animals, and the staff of the University of Tennessee College of Veterinary Medicine (UTCVM) Microbiology Laboratory. This study was funded in part by the UTCVM Center of Excellence Summer Research Program. 2012 B. Lammers, D. Bemis, P. Colclough, and M.J. Souza 63 Literature Cited Bartlett, K.H., T.J. Trust, and H. Lior. 1977. Small pet aquarium frogs as a source of Salmonella. Applied and Environmental Microbiology 33:1026–1029. Barzily, A., and Y. Kott. 1991. Survival of pathogenic bacteria in an adverse envrionment. Water Science and Technology 24:395–400. Baudart, J., K. Lemarchand, A. Brisabois, and P. Lebaron. 2000. Diversity of Salmonella strains isolated from the aquatic environment as determined by serotyping and amplifi cation of the ribosomal DNA spacer regions. Applied and Environmental Microbiology 66:1544–1552. Burnham, B.R., D.H. Atchley, R.P. Defusco, K.E. Ferris, J.C. Zicarelli, J.H. Lee, and F.J. Angulo. 1998. Prevalence of fecal shedding of Salmonella organisms among captive Green Iguanas and potential public health implications. Journal of the American Veterinary Medical Association 213:48–50. Center for Disease Control (CDC). 2009. Multistate outbreak of human Salmonella Typhimurium infections associated with aquatic frogs—US, 2009. MMWR Weekly 58:1433–1436. Chambers, D.L., and A.C. Hulse. 2006. Salmonella serovars in the herpetofauna of Indiana County, Pennsylvania. Applied and Environmental Microbiology 72:3771–3773. Chomel, B.B., A. Belotto, and F.X. Meslin. 2007. Wildlife, exotic pets, and emerging zoonoses. Emerging Infectious Diseases 13:6–11. Clarkson, L.S., M. Tobin-D'angelo, C. Shuler, S. Hanna, J. Benson, and A.C. Voetsch. 2010. Sporadic Salmonella enterica serotype Javiana infections in Georgia and Tennessee: A hypothesis-generating study. Epidemiology and Infection 138:340–346. Everard, C.O.R., B. Tota, D. Bassett, and C. Ali. 1979. Salmonella in Wildlife from Trinidad and Grenada, WI. Journal of Wildlife Diseases 15:213–219. Herikstad, H., Y. Motarjemi, and R.V. Tauxe. 2002. Salmonella surveillance: A global survey of public health serotyping. Epidemiology and Infection 129:1–8. Kourany, M., C.W. Myers, and C.R. Schneider. 1970. Panamanian amphibians and reptiles as carriers of Salmonella. American Journal of Tropical Medicine and Hygiene 19:632–638. Lockhart, J.M., G. Lee, J. Turco, and L. Chamberlin. 2008. Salmonella from Gopher Tortoises (Gopherus polyphemus) in South Georgia. Journal of Wildlife Diseases 44:988–991. Mermin, J., L. Hutwagner, D. Vugia, S. Shallow, P. Daily, J. Bender, J. Koehler, R. Marcus, and F.J. Angulo. 2004. Reptiles, amphibians, and human Salmonella infection: A population-based, case-control study. Clinical Infectious Diseases 38:S253–S261. Morse, E.V., and M.A. Duncan. 1974. Salmonellosis: Environmental health problem. Journal of the American Veterinary Medical Association 165:1015–1019. Nickerson, M., and C. Mays. 1973. The Hellbenders: North American “Giant Salamanders”. Milwaukee Public Museum, Milwaukee, WI. 106 pp. Nickerson, M.A., K.L. Krysko, and R.D. Owen. 2003. Habitat differences affecting age class distributions of the Hellbender salamander, Cryptobranchus alleganiensis. Southeastern Naturalist 2:619–629. Pfleger, S., G. Benyer, R. Sommer, and A. Hassl. 2003. Pattern of Salmonella excretion in amphibians and reptiles in a vivarium. International Journal of Hygiene and Environmental Health 206:53–59. Rosenstein, B.J., P. Russo, and M.C. Hinchliffe. 1965. A family outbreak of salmonellosis traced to a pet turtle New England Journal of Medicine 272:960–961. 64 Southeastern Naturalist Vol. 11, No. 1 Sharma, V.K., Y.K. Kaura, and I.P. Singh. 1974. Frogs as carriers of Salmonella and Edwardsiella. Antonie Van Leeuwenhoek Journal of Microbiology 40:171–175. Srikantiah, P., J.C. Lay, S. Hand, J.A. Crump, J. Campbell, M.S. Van Duyne, R. Bishop, R. Middendor, M. Currier, P.S. Mead, and K. Molbak. 2004. Salmonella enterica serotype Javiana infections associated with amphibian contact, Mississippi, 2001. Epidemiology and Infection 132:273–281. Thomas, A.D., J.C. Forbes-Faulkner, R. Speare, and C. Murray. 2001. Salmonelliasis in Wildlife From Queensland Journal of Wildlife Disease 37:229–238. Trust, T.J., K.H. Bartlett, and H. Lior. 1981. Importation of Salmonellae with aquarium species. Canadian Journal of Microbiology 27:500–504. Van Meervenne, E., N. Botteldoorn, S. Lokietek, M. Vatlet, A. Cupa, M. Naranjo, K. Dierick, and S. Bertrand. 2009. Turtle-associated Salmonella septicaemia and meningitis in a 2-month-old baby. Journal of Medical Microbiology 58:1379–1381. Woodward, D.L., R. Khakhria, and W.M. Johnson. 1997. Human salmonellosis associated with exotic pets. Journal of Clinical Microbiology 35:2786–2790.